This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Filipe, S. R. Articles by Tomasz, A. Search for Related Content PubMed PubMed Citation Articles by Filipe, S. R. Articles by Tomasz, A.

Journal of Bacteriology, December 2000, p. 6798-6805, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Distribution of the Mosaic Structured murM Genes among Natural Populations of Streptococcus pneumoniae

Sergio R. Filipe,^1,2 Elena Severina,^1, and Alexander Tomasz^1,*

Laboratory of Microbiology, The Rockefeller University, New York, New York 10021,¹ and Molecular Genetics Unit, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal²

Received 28 June 2000/Accepted 20 September 2000

The presence and sequence variation of the murM gene were studied in a large collection (814 strains) of genetically diverse Streptococcus pneumoniae isolates, which included 27 different serogroups and both penicillin-resistant (423 isolates, 67 pulsed-field gel electrophoretic [PFGE] types) and intermediately penicillin-resistant (165 isolates, 66 PFGE types) and penicillin-susceptible (226 isolates, 135 PFGE types) strains. Diversity of the murM sequences was tested by hybridization with mainly two kinds of probes: one derived from the amplification of the nucleotide sequence between nucleotides 201 and 624 in the penicillin-susceptible laboratory strain R36A (murMA probe) and a second probe that amplified the comparable, highly divergent sequence in the penicillin-resistant strain Pen6 (murMB probe). The great majority of the strains (761 of 814), including both penicillin-susceptible and penicillin-resistant isolates, reacted exclusively with the murMA probe. A smaller group of penicillin-resistant strains (48 of 814 isolates) reacted only with the murMB DNA probe, and an additional 5 isolates reacted with both probes. High-pressure liquid chromatography analysis of the peptidoglycan of strains hybridizing with murMB showed that they invariably contained an increased proportion of branched peptides. Complete sequencing of murM from a group of penicillin-resistant isolates allowed the identification of a number of different murMB alleles that differed in the length and exact position of the divergent (Pen6 type) sequences within the particular murM. The close similarity of these divergent sequences in the various murM alleles suggests a possible common heterologous origin.

---

^* Corresponding author. Mailing address: Laboratory of Microbiology, The Rockefeller University, 1230 York Ave., New York, NY 10021. Phone: (212) 327-8278. Fax: (212) 327-8688. E-mail: tomasz{at}mail.rockefeller.edu.

Permanent address: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia.

---

Journal of Bacteriology, December 2000, p. 6798-6805, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

• Lloyd, A. J., Gilbey, A. M., Blewett, A. M., De Pascale, G., El Zoeiby, A., Levesque, R. C., Catherwood, A. C., Tomasz, A., Bugg, T. D. H., Roper, D. I., Dowson, C. G. (2008). Characterization of tRNA-dependent Peptide Bond Formation by MurM in the Synthesis of Streptococcus pneumoniae Peptidoglycan. J. Biol. Chem. 283: 6402-6417 [Abstract] [Full Text]  
• del Campo, R., Cafini, F., Morosini, M. I., Fenoll, A., Linares, J., Alou, L., Sevillano, D., Canton, R., Prieto, J., Baquero, F., on behalf of the Spanish Pneumococcal Network (G3/, (2006). Combinations of PBPs and MurM protein variants in early and contemporary high-level penicillin-resistant Streptococcus pneumoniae isolates in Spain. J Antimicrob Chemother 57: 983-986 [Abstract] [Full Text]  
• Cafini, F., del Campo, R., Alou, L., Sevillano, D., Morosini, M. I., Baquero, F., Prieto, J., on behalf of the Spanish Pneumococcal Network (G03, (2006). Alterations of the penicillin-binding proteins and murM alleles of clinical Streptococcus pneumoniae isolates with high-level resistance to amoxicillin in Spain. J Antimicrob Chemother 57: 224-229 [Abstract] [Full Text]  
• Chesnel, L., Carapito, R., Croize, J., Dideberg, O., Vernet, T., Zapun, A. (2005). Identical Penicillin-Binding Domains in Penicillin-Binding Proteins of Streptococcus pneumoniae Clinical Isolates with Different Levels of {beta}-Lactam Resistance. Antimicrob. Agents Chemother. 49: 2895-2902 [Abstract] [Full Text]  
• Rohrer, S., Berger-Bachi, B. (2003). FemABX Peptidyl Transferases: a Link between Branched-Chain Cell Wall Peptide Formation and {beta}-Lactam Resistance in Gram-Positive Cocci. Antimicrob. Agents Chemother. 47: 837-846 [Full Text]  
• Goffin, C., Ghuysen, J.-M. (2002). Biochemistry and Comparative Genomics of SxxK Superfamily Acyltransferases Offer a Clue to the Mycobacterial Paradox: Presence of Penicillin-Susceptible Target Proteins versus Lack of Efficiency of Penicillin as Therapeutic Agent. Microbiol. Mol. Biol. Rev. 66: 702-738 [Abstract] [Full Text]  
• Sa-Leao, R., Tomasz, A., Santos Sanches, I., de Lencastre, H. (2002). Pilot Study of the Genetic Diversity of the Pneumococcal Nasopharyngeal Flora among Children Attending Day Care Centers. J. Clin. Microbiol. 40: 3577-3585 [Abstract] [Full Text]  
• Filipe, S. R., Severina, E., Tomasz, A. (2002). The murMN operon: A functional link between antibiotic resistance and antibiotic tolerance in Streptococcuspneumoniae. Proc. Natl. Acad. Sci. USA 99: 1550-1555 [Abstract] [Full Text]  
• Filipe, S. R., Severina, E., Tomasz, A. (2001). Functional Analysis of Streptococcus pneumoniae MurM Reveals the Region Responsible for Its Specificity in the Synthesis of Branched Cell Wall Peptides. J. Biol. Chem. 276: 39618-39628 [Abstract] [Full Text]