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Journal of Bacteriology, December 2000, p. 6815-6818, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Hexokinase Regulates Kinetics of Glucose Transport and Expression of Genes Encoding Hexose Transporters in Saccharomyces cerevisiae

Thomas Petit,1,2 Jasper A. Diderich,3 Arthur L. Kruckeberg,3,4 Carlos Gancedo,1 and Karel Van Dam3,*

Instituto de Investigaciones Biomédicas Alberto Sols, C.S.I.C.-UAM, 28029 Madrid, Spain,1 and DSM Bakery Ingredients Division, 2600 MA Delft,2 Swammerdam Institute for Life Science, 1018 TV Amsterdam,3 and Department of Molecular Cell Physiology, Free University, 1081 HV Amsterdam,4 The Netherlands

Received 19 May 2000/Accepted 28 August 2000

Glucose transport kinetics and mRNA levels of different glucose transporters were determined in Saccharomyces cerevisiae strains expressing different sugar kinases. During exponential growth on glucose, a hxk2 null strain exhibited high-affinity hexose transport associated with an elevated transcription of the genes HXT2 and HXT7, encoding high-affinity transporters, and a diminished expression of the HXT1 and HXT3 genes, encoding low-affinity transporters. Deletion of HXT7 revealed that the high-affinity component is mostly due to HXT7; however, a previously unidentified very-high-affinity component (Km = 0.19 mM) appeared to be due to other factors. Expression of genes encoding hexokinases from Schizosaccharomyces pombe or Yarrowia lipolytica in a hxk1 hxk2 glk1 strain prevented derepression of the high-affinity transport system at high concentrations of glucose.

* Corresponding author. Mailing address: Swammerdam Institute for Life Science, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands. Phone: 31(20) 525 5510. Fax: 31(20) 525 5505. E-mail: k.van.dam{at}chem.uva.nl.

Journal of Bacteriology, December 2000, p. 6815-6818, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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