This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rachid, S.
Right arrow Articles by Ziebuhr, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rachid, S.
Right arrow Articles by Ziebuhr, W.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2000, p. 6824-6826, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Alternative Transcription Factor sigma B Is Involved in Regulation of Biofilm Expression in a Staphylococcus aureus Mucosal Isolate

Shwan Rachid,1 Knut Ohlsen,1 Ursula Wallner,1 Jörg Hacker,1 Michael Hecker,2 and Wilma Ziebuhr1,*

Institut für Molekulare Infektionsbiologie, D-97070 Würzburg,1 and Institut für Mikrobiologie und Molekularbiologie, 15, D-17487 Greifswald,2 Germany

Received 26 June 2000/Accepted 12 September 2000

Osmotic stress was found to induce biofilm formation in a Staphylococcus aureus mucosal isolate. Inactivation of a global regulator of the bacterial stress response, the alternative transcription factor sigma B, resulted in a biofilm-negative phenotype and loss of salt-induced biofilm production. Complementation of the mutant strain with an expression plasmid encoding sigma B completely restored the wild-type phenotype. The combined data suggest a critical role of sigma B in S. aureus biofilm regulation under environmental stress conditions.

* Corresponding author. Mailing address: Institut für Molekulare Infektionsbiologie, Röntgenring 11, D-97070 Würzburg, Germany. Phone: 49-931- 31 2154. Fax: 49-931- 31 2578. E-mail: w.ziebuhr{at}mail.uni-wuerzburg.de.

Journal of Bacteriology, December 2000, p. 6824-6826, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lauderdale, K. J., Boles, B. R., Cheung, A. L., Horswill, A. R. (2009). Interconnections between Sigma B, agr, and Proteolytic Activity in Staphylococcus aureus Biofilm Maturation. Infect. Immun. 77: 1623-1635 [Abstract] [Full Text]  
  • Cotter, J. J., O'Gara, J. P., Mack, D., Casey, E. (2009). Oxygen-Mediated Regulation of Biofilm Development Is Controlled by the Alternative Sigma Factor {sigma}B in Staphylococcus epidermidis. Appl. Environ. Microbiol. 75: 261-264 [Abstract] [Full Text]  
  • Sadykov, M. R., Olson, M. E., Halouska, S., Zhu, Y., Fey, P. D., Powers, R., Somerville, G. A. (2008). Tricarboxylic Acid Cycle-Dependent Regulation of Staphylococcus epidermidis Polysaccharide Intercellular Adhesin Synthesis. J. Bacteriol. 190: 7621-7632 [Abstract] [Full Text]  
  • Trotonda, M. P., Tamber, S., Memmi, G., Cheung, A. L. (2008). MgrA Represses Biofilm Formation in Staphylococcus aureus. Infect. Immun. 76: 5645-5654 [Abstract] [Full Text]  
  • Cerca, N., Brooks, J. L., Jefferson, K. K. (2008). Regulation of the Intercellular Adhesin Locus Regulator (icaR) by SarA, {sigma}B, and IcaR in Staphylococcus aureus. J. Bacteriol. 190: 6530-6533 [Abstract] [Full Text]  
  • Nagorska, K., Hinc, K., Strauch, M. A., Obuchowski, M. (2008). Influence of the {sigma}B Stress Factor and yxaB, the Gene for a Putative Exopolysaccharide Synthase under {sigma}B Control, on Biofilm Formation. J. Bacteriol. 190: 3546-3556 [Abstract] [Full Text]  
  • Johnson, M., Cockayne, A., Morrissey, J. A. (2008). Iron-Regulated Biofilm Formation in Staphylococcus aureus Newman Requires ica and the Secreted Protein Emp. Infect. Immun. 76: 1756-1765 [Abstract] [Full Text]  
  • Schlag, S., Nerz, C., Birkenstock, T. A., Altenberend, F., Gotz, F. (2007). Inhibition of Staphylococcal Biofilm Formation by Nitrite. J. Bacteriol. 189: 7911-7919 [Abstract] [Full Text]  
  • Zhu, Y., Weiss, E. C., Otto, M., Fey, P. D., Smeltzer, M. S., Somerville, G. A. (2007). Staphylococcus aureus Biofilm Metabolism and the Influence of Arginine on Polysaccharide Intercellular Adhesin Synthesis, Biofilm Formation, and Pathogenesis. Infect. Immun. 75: 4219-4226 [Abstract] [Full Text]  
  • Zhang, X.-S., Garcia-Contreras, R., Wood, T. K. (2007). YcfR (BhsA) Influences Escherichia coli Biofilm Formation through Stress Response and Surface Hydrophobicity. J. Bacteriol. 189: 3051-3062 [Abstract] [Full Text]  
  • Valle, J., Vergara-Irigaray, M., Merino, N., Penades, J. R., Lasa, I. (2007). {sigma}B Regulates IS256-Mediated Staphylococcus aureus Biofilm Phenotypic Variation. J. Bacteriol. 189: 2886-2896 [Abstract] [Full Text]  
  • Tu Quoc, P. H., Genevaux, P., Pajunen, M., Savilahti, H., Georgopoulos, C., Schrenzel, J., Kelley, W. L. (2007). Isolation and Characterization of Biofilm Formation-Defective Mutants of Staphylococcus aureus. Infect. Immun. 75: 1079-1088 [Abstract] [Full Text]  
  • Goldstein, F. (2007). The potential clinical impact of low-level antibiotic resistance in Staphylococcus aureus. J Antimicrob Chemother 59: 1-4 [Abstract] [Full Text]  
  • Shanks, R. M. Q., Sargent, J. L., Martinez, R. M., Graber, M. L., O'Toole, G. A. (2006). Catheter lock solutions influence staphylococcal biofilm formation on abiotic surfaces. Nephrol Dial Transplant 21: 2247-2255 [Abstract] [Full Text]  
  • Alvarez, B., Secades, P., Prieto, M., McBride, M. J., Guijarro, J. A. (2006). A Mutation in Flavobacterium psychrophilum tlpB Inhibits Gliding Motility and Induces Biofilm Formation.. Appl. Environ. Microbiol. 72: 4044-4053 [Abstract] [Full Text]  
  • Mathew, R., Mukherjee, R., Balachandar, R., Chatterji, D. (2006). Deletion of the rpoZ gene, encoding the {omega} subunit of RNA polymerase, results in pleiotropic surface-related phenotypes in Mycobacterium smegmatis. Microbiology 152: 1741-1750 [Abstract] [Full Text]  
  • Viswanathan, P., Singer, M., Kroos, L. (2006). Role of {sigma}D in Regulating Genes and Signals during Myxococcus xanthus Development. J. Bacteriol. 188: 3246-3256 [Abstract] [Full Text]  
  • Kazmierczak, M. J., Wiedmann, M., Boor, K. J. (2005). Alternative Sigma Factors and Their Roles in Bacterial Virulence. Microbiol. Mol. Biol. Rev. 69: 527-543 [Abstract] [Full Text]  
  • Senn, M. M., Giachino, P., Homerova, D., Steinhuber, A., Strassner, J., Kormanec, J., Fluckiger, U., Berger-Bachi, B., Bischoff, M. (2005). Molecular Analysis and Organization of the {sigma}B Operon in Staphylococcus aureus. J. Bacteriol. 187: 8006-8019 [Abstract] [Full Text]  
  • Johnson, M., Cockayne, A., Williams, P. H., Morrissey, J. A. (2005). Iron-Responsive Regulation of Biofilm Formation in Staphylococcus aureus Involves Fur-Dependent and Fur-Independent Mechanisms. J. Bacteriol. 187: 8211-8215 [Abstract] [Full Text]  
  • Trotonda, M. P., Manna, A. C., Cheung, A. L., Lasa, I., Penades, J. R. (2005). SarA Positively Controls Bap-Dependent Biofilm Formation in Staphylococcus aureus. J. Bacteriol. 187: 5790-5798 [Abstract] [Full Text]  
  • Shanks, R. M. Q., Donegan, N. P., Graber, M. L., Buckingham, S. E., Zegans, M. E., Cheung, A. L., O'Toole, G. A. (2005). Heparin Stimulates Staphylococcus aureus Biofilm Formation. Infect. Immun. 73: 4596-4606 [Abstract] [Full Text]  
  • Toledo-Arana, A., Merino, N., Vergara-Irigaray, M., Debarbouille, M., Penades, J. R., Lasa, I. (2005). Staphylococcus aureus Develops an Alternative, ica-Independent Biofilm in the Absence of the arlRS Two-Component System. J. Bacteriol. 187: 5318-5329 [Abstract] [Full Text]  
  • Vuong, C., Kidder, J. B., Jacobson, E. R., Otto, M., Proctor, R. A., Somerville, G. A. (2005). Staphylococcus epidermidis Polysaccharide Intercellular Adhesin Production Significantly Increases during Tricarboxylic Acid Cycle Stress. J. Bacteriol. 187: 2967-2973 [Abstract] [Full Text]  
  • Zhang, H., Morikawa, K., Ohta, T., Kato, Y. (2005). In vitro resistance to the CS{alpha}{beta}-type antimicrobial peptide ASABF- is conferred by overexpression of sigma factor sigB in Staphylococcus aureus. J Antimicrob Chemother 55: 686-691 [Abstract] [Full Text]  
  • Streker, K., Freiberg, C., Labischinski, H., Hacker, J., Ohlsen, K. (2005). Staphylococcus aureus NfrA (SA0367) Is a Flavin Mononucleotide-Dependent NADPH Oxidase Involved in Oxidative Stress Response. J. Bacteriol. 187: 2249-2256 [Abstract] [Full Text]  
  • Tormo, M. A., Marti, M., Valle, J., Manna, A. C., Cheung, A. L., Lasa, I., Penades, J. R. (2005). SarA Is an Essential Positive Regulator of Staphylococcus epidermidis Biofilm Development. J. Bacteriol. 187: 2348-2356 [Abstract] [Full Text]  
  • Knobloch, J. K.-M., Jager, S., Huck, J., Horstkotte, M. A., Mack, D. (2005). mecA Is Not Involved in the {sigma}B-Dependent Switch of the Expression Phenotype of Methicillin Resistance in Staphylococcus epidermidis. Antimicrob. Agents Chemother. 49: 1216-1219 [Abstract] [Full Text]  
  • Fluckiger, U., Ulrich, M., Steinhuber, A., Doring, G., Mack, D., Landmann, R., Goerke, C., Wolz, C. (2005). Biofilm Formation, icaADBC Transcription, and Polysaccharide Intercellular Adhesin Synthesis by Staphylococci in a Device-Related Infection Model. Infect. Immun. 73: 1811-1819 [Abstract] [Full Text]  
  • Kennedy, C. A, O'Gara, J. P (2004). Contribution of culture media and chemical properties of polystyrene tissue culture plates to biofilm development by Staphylococcus aureus. J Med Microbiol 53: 1171-1173 [Full Text]  
  • Jonsson, I.-M., Arvidson, S., Foster, S., Tarkowski, A. (2004). Sigma Factor B and RsbU Are Required for Virulence in Staphylococcus aureus-Induced Arthritis and Sepsis. Infect. Immun. 72: 6106-6111 [Abstract] [Full Text]  
  • Conlon, K. M., Humphreys, H., O'Gara, J. P. (2004). Inactivations of rsbU and sarA by IS256 Represent Novel Mechanisms of Biofilm Phenotypic Variation in Staphylococcus epidermidis. J. Bacteriol. 186: 6208-6219 [Abstract] [Full Text]  
  • Beenken, K. E., Dunman, P. M., McAleese, F., Macapagal, D., Murphy, E., Projan, S. J., Blevins, J. S., Smeltzer, M. S. (2004). Global Gene Expression in Staphylococcus aureus Biofilms. J. Bacteriol. 186: 4665-4684 [Abstract] [Full Text]  
  • Knobloch, J. K.-M., Jager, S., Horstkotte, M. A., Rohde, H., Mack, D. (2004). RsbU-Dependent Regulation of Staphylococcus epidermidis Biofilm Formation Is Mediated via the Alternative Sigma Factor {sigma}B by Repression of the Negative Regulator Gene icaR. Infect. Immun. 72: 3838-3848 [Abstract] [Full Text]  
  • Bischoff, M., Dunman, P., Kormanec, J., Macapagal, D., Murphy, E., Mounts, W., Berger-Bachi, B., Projan, S. (2004). Microarray-Based Analysis of the Staphylococcus aureus {sigma}B Regulon. J. Bacteriol. 186: 4085-4099 [Abstract] [Full Text]  
  • Jefferson, K. K., Pier, D. B., Goldmann, D. A., Pier, G. B. (2004). The Teicoplanin-Associated Locus Regulator (TcaR) and the Intercellular Adhesin Locus Regulator (IcaR) Are Transcriptional Inhibitors of the ica Locus in Staphylococcus aureus. J. Bacteriol. 186: 2449-2456 [Abstract] [Full Text]  
  • Lim, Y., Jana, M., Luong, T. T., Lee, C. Y. (2004). Control of Glucose- and NaCl-Induced Biofilm Formation by rbf in Staphylococcus aureus. J. Bacteriol. 186: 722-729 [Abstract] [Full Text]  
  • Uziel, O., Borovok, I., Schreiber, R., Cohen, G., Aharonowitz, Y. (2004). Transcriptional Regulation of the Staphylococcus aureus Thioredoxin and Thioredoxin Reductase Genes in Response to Oxygen and Disulfide Stress. J. Bacteriol. 186: 326-334 [Abstract] [Full Text]  
  • Kristich, C. J., Li, Y.-H., Cvitkovitch, D. G., Dunny, G. M. (2004). Esp-Independent Biofilm Formation by Enterococcus faecalis. J. Bacteriol. 186: 154-163 [Abstract] [Full Text]  
  • Wu, J. A., Kusuma, C., Mond, J. J., Kokai-Kun, J. F. (2003). Lysostaphin Disrupts Staphylococcus aureus and Staphylococcus epidermidis Biofilms on Artificial Surfaces. Antimicrob. Agents Chemother. 47: 3407-3414 [Abstract] [Full Text]  
  • Knobloch, J. K.-M., Nedelmann, M., Kiel, K., Bartscht, K., Horstkotte, M. A., Dobinsky, S., Rohde, H., Mack, D. (2003). Establishment of an Arbitrary PCR for Rapid Identification of Tn917 Insertion Sites in Staphylococcus epidermidis: Characterization of Biofilm-Negative and Nonmucoid Mutants. Appl. Environ. Microbiol. 69: 5812-5818 [Abstract] [Full Text]  
  • Kazmierczak, M. J., Mithoe, S. C., Boor, K. J., Wiedmann, M. (2003). Listeria monocytogenes {sigma}B Regulates Stress Response and Virulence Functions. J. Bacteriol. 185: 5722-5734 [Abstract] [Full Text]  
  • Moretro, T., Hermansen, L., Holck, A. L., Sidhu, M. S., Rudi, K., Langsrud, S. (2003). Biofilm Formation and the Presence of the Intercellular Adhesion Locus ica among Staphylococci from Food and Food Processing Environments. Appl. Environ. Microbiol. 69: 5648-5655 [Abstract] [Full Text]  
  • Beenken, K. E., Blevins, J. S., Smeltzer, M. S. (2003). Mutation of sarA in Staphylococcus aureus Limits Biofilm Formation. Infect. Immun. 71: 4206-4211 [Abstract] [Full Text]  
  • Dobinsky, S., Kiel, K., Rohde, H., Bartscht, K., Knobloch, J. K.-M., Horstkotte, M. A., Mack, D. (2003). Glucose-Related Dissociation between icaADBC Transcription and Biofilm Expression by Staphylococcus epidermidis: Evidence for an Additional Factor Required for Polysaccharide Intercellular Adhesin Synthesis. J. Bacteriol. 185: 2879-2886 [Abstract] [Full Text]  
  • Sifri, C. D., Begun, J., Ausubel, F. M., Calderwood, S. B. (2003). Caenorhabditis elegans as a Model Host for Staphylococcus aureus Pathogenesis. Infect. Immun. 71: 2208-2217 [Abstract] [Full Text]  
  • Price, C. T. D., Singh, V. K., Jayaswal, R. K., Wilkinson, B. J., Gustafson, J. E. (2002). Pine Oil Cleaner-Resistant Staphylococcus aureus: Reduced Susceptibility to Vancomycin and Oxacillin and Involvement of SigB. Appl. Environ. Microbiol. 68: 5417-5421 [Abstract] [Full Text]  
  • Horsburgh, M. J., Aish, J. L., White, I. J., Shaw, L., Lithgow, J. K., Foster, S. J. (2002). {sigma}B Modulates Virulence Determinant Expression and Stress Resistance: Characterization of a Functional rsbU Strain Derived from Staphylococcus aureus 8325-4. J. Bacteriol. 184: 5457-5467 [Abstract] [Full Text]  
  • Oosthuizen, M. C., Steyn, B., Theron, J., Cosette, P., Lindsay, D., von Holy, A., Brozel, V. S. (2002). Proteomic Analysis Reveals Differential Protein Expression by Bacillus cereus during Biofilm Formation. Appl. Environ. Microbiol. 68: 2770-2780 [Abstract] [Full Text]  
  • Sakoulas, G., Eliopoulos, G. M., Moellering, R. C. Jr., Wennersten, C., Venkataraman, L., Novick, R. P., Gold, H. S. (2002). Accessory Gene Regulator (agr) Locus in Geographically Diverse Staphylococcus aureus Isolates with Reduced Susceptibility to Vancomycin. Antimicrob. Agents Chemother. 46: 1492-1502 [Abstract] [Full Text]  
  • Bateman, B. T., Donegan, N. P., Jarry, T. M., Palma, M., Cheung, A. L. (2001). Evaluation of a Tetracycline-Inducible Promoter in Staphylococcus aureus In Vitro and In Vivo and Its Application in Demonstrating the Role of sigB in Microcolony Formation. Infect. Immun. 69: 7851-7857 [Abstract] [Full Text]  
  • Palma, M., Cheung, A. L. (2001). sigma B Activity in Staphylococcus aureus Is Controlled by RsbU and an Additional Factor(s) during Bacterial Growth. Infect. Immun. 69: 7858-7865 [Abstract] [Full Text]  
  • Kies, S., Otto, M., Vuong, C., Gotz, F. (2001). Identification of the sigB Operon in Staphylococcus epidermidis: Construction and Characterization of a sigB Deletion Mutant. Infect. Immun. 69: 7933-7936 [Abstract] [Full Text]  
  • Rohde, H., Knobloch, J. K. M., Horstkotte, M. A., Mack ;, D., Arciola, C. R., Montanaro, L., Baldassarri, L. (2001). Correlation of Staphylococcus aureus icaADBC Genotype and Biofilm Expression Phenotype. J. Clin. Microbiol. 39: 4595-4596 [Full Text]  
  • Knobloch, J. K.-M., Bartscht, K., Sabottke, A., Rohde, H., Feucht, H.-H., Mack, D. (2001). Biofilm Formation by Staphylococcus epidermidis Depends on Functional RsbU, an Activator of the sigB Operon: Differential Activation Mechanisms Due to Ethanol and Salt Stress. J. Bacteriol. 183: 2624-2633 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»