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Journal of Bacteriology, December 2000, p. 6831-6833, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Interspecies Complementation of Escherichia coli ccm Mutants: CcmE (CycJ) from Bradyrhizobium japonicum Acts as a Heme Chaperone during Cytochrome c Maturation

Henk Schulz and Linda Thöny-Meyer*

Institut für Mikrobiologie, Eidgenössische Technische Hochschule, CH 8092 Zürich, Switzerland

Received 18 July 2000/Accepted 10 September 2000

Biogenesis of c-type cytochromes in alpha - and gamma -proteobacteria requires the function of a set of orthologous genes (ccm genes) that encode specific maturation factors. The Escherichia coli CcmE protein is a periplasmic heme chaperone. The membrane protein CcmC is required for loading CcmE with heme. By expressing CcmE (CycJ) from Bradyrhizobium japonicum in E. coli we demonstrated that heme is bound covalently to this protein at a strictly conserved histidine residue. The B. japonicum homologue can transfer heme to apocytochrome c in E. coli, suggesting that it functions as a heme chaperone. CcmC (CycZ) from B. japonicum expressed in E. coli was capable of inserting heme into CcmE.

* Corresponding author. Mailing address: Institut für Mikrobiologie, Eidgenössische Technische Hochschule, Schmelzbergstrasse 7, CH 8092 Zürich, Switzerland. Phone: 41-1-632 3326. Fax: 41-1-632 1148. E-mail: lthoeny{at}micro.biol.ethz.ch.

Journal of Bacteriology, December 2000, p. 6831-6833, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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