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Journal of Bacteriology, December 2000, p. 6834-6841, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Minimal and Contributing Sequence Determinants of the cis-Acting Locus of Transfer (clt) of Streptomycete Plasmid pIJ101 Occur within an Intrinsically Curved Plasmid Region

Matthew J. Ducote, Shubha Prakash,dagger and Gregg S. Pettis*

Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803

Received 13 July 2000/Accepted 6 September 2000

Efficient interbacterial transfer of streptomycete plasmid pIJ101 requires the pIJ101 tra gene, as well as a cis-acting plasmid function known as clt. Here we show that the minimal pIJ101 clt locus consists of a sequence no greater than 54 bp in size that includes essential inverted-repeat and direct-repeat sequences and is located in close proximity to the 3' end of the korB regulatory gene. Evidence that sequences extending beyond the minimal locus and into the korB open reading frame influence clt transfer function and demonstration that clt-korB sequences are intrinsically curved raise the possibility that higher-order structuring of DNA and protein within this plasmid region may be an inherent feature of efficient pIJ101 transfer.

* Corresponding author. Mailing address: Department of Biological Sciences, Louisiana State University, 508 Life Sciences Bldg., Baton Rouge, LA 70803. Phone: (225) 388-2798. Fax: (225) 388-2597. E-mail: gpettis{at}unix1.sncc.lsu.edu.

dagger Present address: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India.

Journal of Bacteriology, December 2000, p. 6834-6841, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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