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Journal of Bacteriology, December 2000, p. 6857-6861, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Expression of cbsA Encoding the Collagen-Binding S-Protein of Lactobacillus crispatus JCM5810 in Lactobacillus casei ATCC 393T

Beatriz Martínez,1,2,dagger Jouko Sillanpää,3 Egbert Smit,1 Timo K. Korhonen,3 and Peter H. Pouwels1,2,*

Department of Applied Microbiology and Gene Technology, TNO Voeding, 3700 AJ Zeist,1 and Wageningen Centre for Food Sciences, 6700 AN Wageningen,2 The Netherlands, and Department of Biosciences, Division of General Microbiology, FIN-00014 University of Helsinki, Finland3

Received 25 May 2000/Accepted 19 September 2000

The cbsA gene encoding the collagen-binding S-layer protein of Lactobacillus crispatus JCM5810 was expressed in L. casei ATCC 393T. The S-protein was not retained on the surface of the recombinant bacteria but was secreted into the medium. By translational fusion of CbsA to the cell wall sorting signal of the proteinase, PrtP, of L. casei, CbsA was presented at the surface, rendering the transformants able to bind to immobilized collagens.

* Corresponding author. Mailing address: Department of Applied Microbiology and Gene Technology, TNO Voeding, P.O. Box 360, 3700 AJ Zeist, The Netherlands. Phone: 31 (0) 30 6944462. Fax: 31 (0) 30 6944466. E-mail: Pouwels{at}voeding.tno.nl.

dagger Present address: Instituto de Productos Lácteos de Asturias, CSIC Apdo. 85. 33300 Villaviciosa, Spain.

Journal of Bacteriology, December 2000, p. 6857-6861, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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