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Journal of Bacteriology, December 2000, p. 6906-6912, Vol. 182, No. 24
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Effects of Major Spore-Specific DNA Binding Proteins on Bacillus subtilis Sporulation and Spore Properties

Barbara Setlow, Kelly A. McGinnis, Katerina Ragkousi, and Peter Setlow*

Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032

Received 10 July 2000/Accepted 4 October 2000

Sporulation of a Bacillus subtilis strain (termed alpha - beta -) lacking the majority of the alpha /beta -type small, acid-soluble spore proteins (SASP) that are synthesized in the developing forespore and saturate spore DNA exhibited a number of differences from that of the wild-type strain, including delayed forespore accumulation of dipicolinic acid, overexpression of forespore-specific genes, and delayed expression of at least one mother cell-specific gene turned on late in sporulation, although genes turned on earlier in the mother cell were expressed normally in alpha - beta - strains. The sporulation defects in alpha - beta - strains were corrected by synthesis of chromosome-saturating levels of either of two wild-type, alpha /beta -type SASP but not by a mutant SASP that binds DNA poorly. Spores from alpha - beta - strains also exhibited less glutaraldehyde resistance and slower outgrowth than did wild-type spores, but at least some of these defects in alpha - beta - spores were abolished by the synthesis of normal levels of alpha /beta -type SASP. These results indicate that alpha /beta -type SASP may well have global effects on gene expression during sporulation and spore outgrowth.

* Corresponding author. Mailing address: Department of Biochemistry MC-3305, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT 06032. Phone: (860) 679-2607. Fax: (860) 679-3408. E-mail: setlow{at}sun.uchc.edu.

Journal of Bacteriology, December 2000, p. 6906-6912, Vol. 182, No. 24
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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