Metabolism of Acyl-Homoserine Lactone Quorum-Sensing Signals by Variovorax paradoxus

doi:10.1128/JB.182.24.6921-6926.2000

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Journal of Bacteriology, December 2000, p. 6921-6926, Vol. 182, No. 24
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Metabolism of Acyl-Homoserine Lactone Quorum-Sensing Signals by Variovorax paradoxus

Jared R. Leadbetter and E. P. Greenberg^*

Department of Microbiology, University of Iowa, Iowa City, Iowa 52242

Received 19 May 2000/Accepted 27 September 2000

Acyl-homoserine lactones (acyl-HSLs) serve as dedicated cell-to-cell signaling molecules in many species of the class Proteobacteria.We have addressed the question of whether these compounds canbe degraded biologically. A motile, rod-shaped bacterium was isolatedfrom soil based upon its ability to utilize N-(3-oxohexanoyl)-L-homoserinelactone as the sole source of energy and nitrogen. The bacteriumwas classified as a strain of Variovorax paradoxus. The V. paradoxusisolate was capable of growth on all of the acyl-HSLs tested.The molar growth yields correlated with the length of the acylgroup. HSL, a product of acyl-HSL metabolism, was used as a nitrogensource, but not as an energy source. Cleavage and partial mineralizationof the HSL ring were demonstrated by using radiolabeled substrate.This study indicates that some strains of V. paradoxus degradeand grow on acyl-HSL signals as the sole energy and nitrogen sources.This study provides clues about the metabolic pathway of acyl-HSLdegradation by V. paradoxus.

^* Corresponding author. Mailing address: Department of Microbiology, University of Iowa, Iowa City, IA 52242. Phone: (319) 335-7990.Fax: (319) 335-7949. E-mail: epgreen{at}blue.weeg.uiowa.edu.

Present address: California Institute of Technology, Pasadena, CA91125.

Journal of Bacteriology, December 2000, p. 6921-6926, Vol. 182, No. 24
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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