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Journal of Bacteriology, December 2000, p. 6921-6926, Vol. 182, No. 24
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Metabolism of Acyl-Homoserine Lactone Quorum-Sensing Signals by Variovorax paradoxus

Jared R. Leadbetterdagger and E. P. Greenberg*

Department of Microbiology, University of Iowa, Iowa City, Iowa 52242

Received 19 May 2000/Accepted 27 September 2000

Acyl-homoserine lactones (acyl-HSLs) serve as dedicated cell-to-cell signaling molecules in many species of the class Proteobacteria. We have addressed the question of whether these compounds can be degraded biologically. A motile, rod-shaped bacterium was isolated from soil based upon its ability to utilize N-(3-oxohexanoyl)-L-homoserine lactone as the sole source of energy and nitrogen. The bacterium was classified as a strain of Variovorax paradoxus. The V. paradoxus isolate was capable of growth on all of the acyl-HSLs tested. The molar growth yields correlated with the length of the acyl group. HSL, a product of acyl-HSL metabolism, was used as a nitrogen source, but not as an energy source. Cleavage and partial mineralization of the HSL ring were demonstrated by using radiolabeled substrate. This study indicates that some strains of V. paradoxus degrade and grow on acyl-HSL signals as the sole energy and nitrogen sources. This study provides clues about the metabolic pathway of acyl-HSL degradation by V. paradoxus.

* Corresponding author. Mailing address: Department of Microbiology, University of Iowa, Iowa City, IA 52242. Phone: (319) 335-7990. Fax: (319) 335-7949. E-mail: epgreen{at}blue.weeg.uiowa.edu.

dagger Present address: California Institute of Technology, Pasadena, CA 91125.

Journal of Bacteriology, December 2000, p. 6921-6926, Vol. 182, No. 24
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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