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Journal of Bacteriology, December 2000, p. 7070-7074, Vol. 182, No. 24
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

High-Frequency Flp Recombinase-Mediated Inversions of the oriC-Containing Region of the Pseudomonas aeruginosa Genome

Nazir Barekzi,1 Kerry Beinlich,1 Tung T. Hoang,1,dagger Xuan-Quynh Pham,2 RoxAnn Karkhoff-Schweizer,1 and Herbert P. Schweizer1,*

Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523,1 and University of Washington Genome Center, Seattle, Washington 981952

Received 30 May 2000/Accepted 2 October 2000

The genomes of the two clonally derived Pseudomonas aeruginosa prototypic strains PAO1 and DSM-1707 differ by the presence of a 2.19-Mb inversion including oriC. Integration of two Flp recombinase target sites near the rrn operons containing the inversion endpoints in PAO1 led to Flp-catalyzed inversion of the intervening 1.59-Mb fragment, including oriC, at high frequencies (83%), favoring the chromosome configuration found in DSM-1707. The results indicate that the oriC-containing region of the P. aeruginosa chromosome can readily undergo and tolerate large inversions.

* Corresponding author. Mailing address: Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1677. Phone: (970) 491-3536. Fax: (970) 491-1815. E-mail: hschweiz{at}cvmbs.colostate.edu.

dagger Present address: Department of Biological Sciences, University of Calgary, Calgary, Alberta T4N 2N1, Canada.

Journal of Bacteriology, December 2000, p. 7070-7074, Vol. 182, No. 24
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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