Characterization of a Major Cluster of nif, fix, and Associated Genes in a Sugarcane Endophyte, Acetobacter diazotrophicus

doi:10.1128/JB.182.24.7088-7091.2000

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Journal of Bacteriology, December 2000, p. 7088-7091, Vol. 182, No. 24
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of a Major Cluster of nif, fix, and Associated Genes in a Sugarcane Endophyte, Acetobacter diazotrophicus

Sunhee Lee,¹ Alexander Reth,² Dietmar Meletzus,² Myrna Sevilla,¹ and Christina Kennedy¹^,^*

Department of Plant Pathology, University of Arizona, Tucson, Arizona,¹ and Department of Gene Technology and Microbiology, Faculty of Biology, University of Bielefeld, 33501, Bielefeld, Germany²

Received 5 June 2000/Accepted 23 September 2000

A major 30.5-kb cluster of nif and associated genes of Acetobacter diazotrophicus (syn. Gluconacetobacter diazotrophicus),a nitrogen-fixing endophyte of sugarcane, was sequenced and analyzed.This cluster represents the largest assembly of contiguous nif-fixand associated genes so far characterized in any diazotrophicbacterial species. Northern blots and promoter sequence analysisindicated that the genes are organized into eight transcriptionalunits. The overall arrangement of genes is most like that of thenif-fix cluster in Azospirillum brasilense, while the individualgene products are more similar to those in species of Rhizobiaceaeor in Rhodobacter capsulatus.

^* Corresponding author. Mailing address: Department of Plant Pathology, Forbes 204, University of Arizona, Tueson, AZ 85721.Phone: (520) 621-9835. Fax: (520) 621-9290. E-mail: ckennedy{at}u.arizona.edu.

Journal of Bacteriology, December 2000, p. 7088-7091, Vol. 182, No. 24
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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