This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Enz, S.
Right arrow Articles by Braun, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Enz, S.
Right arrow Articles by Braun, V.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2000, p. 637-646, Vol. 182, No. 3
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Surface Signaling in Ferric Citrate Transport Gene Induction: Interaction of the FecA, FecR, and FecI Regulatory Proteins

Sabine Enz, Susanne Mahren, Uwe H. Stroeher, and Volkmar Braun*

Mikrobiologie/Membranphysiologie, Universität Tübingen, D-72076 Tübingen, Germany

Received 28 July 1999/Accepted 26 October 1999

In Escherichia coli, transcription of the ferric citrate transport genes fecABCDE is controlled by a novel signal transduction mechanism that starts at the cell surface. Binding of ferric citrate to the outer membrane protein FecA initiates a signal that is transmitted by FecR across the cytoplasmic membrane into the cytoplasm where FecI, the sigma factor, is activated. Interaction between the signaling proteins was demonstrated by utilizing two methods. In in vitro binding assays, FecR that was His tagged at the N terminus [(His)10-FecR] and bound to a Ni-nitrilotriacetic acid agarose column was able to retain FecA, and FecR that was His tagged at the C terminus [FecR-(His)6] retained FecI on the column. An N-terminally truncated, induction-negative but transport-active FecA protein did not bind to (His)10-FecR. The in vivo assay involved the determination of the FecA, FecR, and FecI interacting domains with the bacterial two-hybrid Lex-based system. FecA1-79 interacts with FecR101-317 and FecR1-85 interacts with FecI1-173. These data clearly support a model that proposes interaction of the periplasmic N terminus of FecA with the periplasmic C-terminal portion of FecR and interaction of the cytoplasmic N terminus of FecR with FecI, which results in FecI activation.

* Corresponding author. Mailing address: Mikrobiologie/Membranphysiologie, Universität Tübingen, Auf der Morgenstelle 28, D-72076 Tübingen, Germany. Phone: 49 7071 2972096. Fax: 49 7071 294634. E-mail: volkmar.braun{at}microbiol.uni-tuebingen.de.

Journal of Bacteriology, February 2000, p. 637-646, Vol. 182, No. 3
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Miethke, M., Marahiel, M. A. (2007). Siderophore-Based Iron Acquisition and Pathogen Control. Microbiol. Mol. Biol. Rev. 71: 413-451 [Abstract] [Full Text]  
  • Huang, T.-P., Wong, A. C. L. (2007). A Cyclic AMP Receptor Protein-Regulated Cell-Cell Communication System Mediates Expression of a FecA Homologue in Stenotrophomonas maltophilia. Appl. Environ. Microbiol. 73: 5034-5040 [Abstract] [Full Text]  
  • Ferguson, A. D., Amezcua, C. A., Halabi, N. M., Chelliah, Y., Rosen, M. K., Ranganathan, R., Deisenhofer, J. (2007). Signal transduction pathway of TonB-dependent transporters. Proc. Natl. Acad. Sci. USA 104: 513-518 [Abstract] [Full Text]  
  • Diavatopoulos, D. A., Cummings, C. A., van der Heide, H. G. J., van Gent, M., Liew, S., Relman, D. A., Mooi, F. R. (2006). Characterization of a Highly Conserved Island in the Otherwise Divergent Bordetella holmesii and Bordetella pertussis Genomes. J. Bacteriol. 188: 8385-8394 [Abstract] [Full Text]  
  • Breidenstein, E., Mahren, S., Braun, V. (2006). Residues Involved in FecR Binding Are Localized on One Side of the FecA Signaling Domain in Escherichia coli.. J. Bacteriol. 188: 6440-6442 [Abstract] [Full Text]  
  • Redly, G. A., Poole, K. (2005). FpvIR Control of fpvA Ferric Pyoverdine Receptor Gene Expression in Pseudomonas aeruginosa: Demonstration of an Interaction between FpvI and FpvR and Identification of Mutations in Each Compromising This Interaction. J. Bacteriol. 187: 5648-5657 [Abstract] [Full Text]  
  • James, H. E., Beare, P. A., Martin, L. W., Lamont, I. L. (2005). Mutational Analysis of a Bifunctional Ferrisiderophore Receptor and Signal-Transducing Protein from Pseudomonas aeruginosa. J. Bacteriol. 187: 4514-4520 [Abstract] [Full Text]  
  • Letoffe, S., Wecker, K., Delepierre, M., Delepelaire, P., Wandersman, C. (2005). Activities of the Serratia marcescens Heme Receptor HasR and Isolated Plug and {beta}-Barrel Domains: the {beta}-Barrel Forms a Heme-Specific Channel. J. Bacteriol. 187: 4637-4645 [Abstract] [Full Text]  
  • King, N. D., Kirby, A. E., Connell, T. D. (2005). Transcriptional Control of the rhuIR-bhuRSTUV Heme Acquisition Locus in Bordetella avium. Infect. Immun. 73: 1613-1624 [Abstract] [Full Text]  
  • Susin, M. F., Perez, H. R., Baldini, R. L., Gomes, S. L. (2004). Functional and Structural Analysis of HrcA Repressor Protein from Caulobacter crescentus. J. Bacteriol. 186: 6759-6767 [Abstract] [Full Text]  
  • Alegria, M. C., Docena, C., Khater, L., Ramos, C. H. I., da Silva, A. C. R., Farah, C. S. (2004). New Protein-Protein Interactions Identified for the Regulatory and Structural Components and Substrates of the Type III Secretion System of the Phytopathogen Xanthomonas axonopodis Pathovar citri. J. Bacteriol. 186: 6186-6197 [Abstract] [Full Text]  
  • Sauter, A., Braun, V. (2004). Defined Inactive FecA Derivatives Mutated in Functional Domains of the Outer Membrane Transport and Signaling Protein of Escherichia coli K-12. J. Bacteriol. 186: 5303-5310 [Abstract] [Full Text]  
  • Yoshimura, M., Asai, K., Sadaie, Y., Yoshikawa, H. (2004). Interaction of Bacillus subtilis extracytoplasmic function (ECF) sigma factors with the N-terminal regions of their potential anti-sigma factors. Microbiology 150: 591-599 [Abstract] [Full Text]  
  • Kirby, A. E., King, N. D., Connell, T. D. (2004). RhuR, an Extracytoplasmic Function Sigma Factor Activator, Is Essential for Heme-Dependent Expression of the Outer Membrane Heme and Hemoprotein Receptor of Bordetella avium. Infect. Immun. 72: 896-907 [Abstract] [Full Text]  
  • Enz, S., Brand, H., Orellana, C., Mahren, S., Braun, V. (2003). Sites of Interaction between the FecA and FecR Signal Transduction Proteins of Ferric Citrate Transport in Escherichia coli K-12. J. Bacteriol. 185: 3745-3752 [Abstract] [Full Text]  
  • Chain, P., Lamerdin, J., Larimer, F., Regala, W., Lao, V., Land, M., Hauser, L., Hooper, A., Klotz, M., Norton, J., Sayavedra-Soto, L., Arciero, D., Hommes, N., Whittaker, M., Arp, D. (2003). Complete Genome Sequence of the Ammonia-Oxidizing Bacterium and Obligate Chemolithoautotroph Nitrosomonas europaea. J. Bacteriol. 185: 2759-2773 [Abstract] [Full Text]  
  • Enz, S., Mahren, S., Menzel, C., Braun, V. (2003). Analysis of the Ferric Citrate Transport Gene Promoter of Escherichia coli. J. Bacteriol. 185: 2387-2391 [Abstract] [Full Text]  
  • Mahren, S., Braun, V. (2003). The FecI Extracytoplasmic-Function Sigma Factor of Escherichia coli Interacts with the {beta}' Subunit of RNA Polymerase. J. Bacteriol. 185: 1796-1802 [Abstract] [Full Text]  
  • Ogierman, M., Braun, V. (2003). Interactions between the Outer Membrane Ferric Citrate Transporter FecA and TonB: Studies of the FecA TonB Box. J. Bacteriol. 185: 1870-1885 [Abstract] [Full Text]  
  • Vanderpool, C. K., Armstrong, S. K. (2003). Heme-Responsive Transcriptional Activation of Bordetella bhu Genes. J. Bacteriol. 185: 909-917 [Abstract] [Full Text]  
  • Burton, C. L., Chhabra, S. R., Swift, S., Baldwin, T. J., Withers, H., Hill, S. J., Williams, P. (2002). The Growth Response of Escherichia coli to Neurotransmitters and Related Catecholamine Drugs Requires a Functional Enterobactin Biosynthesis and Uptake System. Infect. Immun. 70: 5913-5923 [Abstract] [Full Text]  
  • Kiratisin, P., Tucker, K. D., Passador, L. (2002). LasR, a Transcriptional Activator of Pseudomonas aeruginosa Virulence Genes, Functions as a Multimer. J. Bacteriol. 184: 4912-4919 [Abstract] [Full Text]  
  • Reischl, S., Wiegert, T., Schumann, W. (2002). Isolation and Analysis of Mutant Alleles of the Bacillus subtilis HrcA Repressor with Reduced Dependency on GroE Function. J. Biol. Chem. 277: 32659-32667 [Abstract] [Full Text]  
  • Mahren, S., Enz, S., Braun, V. (2002). Functional Interaction of Region 4 of the Extracytoplasmic Function Sigma Factor FecI with the Cytoplasmic Portion of the FecR Transmembrane Protein of the Escherichia coli Ferric Citrate Transport System. J. Bacteriol. 184: 3704-3711 [Abstract] [Full Text]  
  • Shen, J., Meldrum, A., Poole, K. (2002). FpvA Receptor Involvement in Pyoverdine Biosynthesis in Pseudomonas aeruginosa. J. Bacteriol. 184: 3268-3275 [Abstract] [Full Text]  
  • Lamont, I. L., Beare, P. A., Ochsner, U., Vasil, A. I., Vasil, M. L. (2002). Siderophore-mediated signaling regulates virulence factor production in Pseudomonasaeruginosa. Proc. Natl. Acad. Sci. USA 99: 7072-7077 [Abstract] [Full Text]  
  • Howard, S. P., Herrmann, C., Stratilo, C. W., Braun, V. (2001). In Vivo Synthesis of the Periplasmic Domain of TonB Inhibits Transport through the FecA and FhuA Iron Siderophore Transporters of Escherichia coli. J. Bacteriol. 183: 5885-5895 [Abstract] [Full Text]  
  • Vanderpool, C. K., Armstrong, S. K. (2001). The Bordetella bhu Locus Is Required for Heme Iron Utilization. J. Bacteriol. 183: 4278-4287 [Abstract] [Full Text]  
  • Pradel, E., Locht, C. (2001). Expression of the Putative Siderophore Receptor Gene bfrZ Is Controlled by the Extracytoplasmic-Function Sigma Factor BupI in Bordetella bronchiseptica. J. Bacteriol. 183: 2910-2917 [Abstract] [Full Text]  
  • Stiefel, A., Mahren, S., Ochs, M., Schindler, P. T., Enz, S., Braun, V. (2001). Control of the Ferric Citrate Transport System of Escherichia coli: Mutations in Region 2.1 of the FecI Extracytoplasmic-Function Sigma Factor Suppress Mutations in the FecR Transmembrane Regulatory Protein. J. Bacteriol. 183: 162-170 [Abstract] [Full Text]  
  • McClelland, M., Florea, L., Sanderson, K., Clifton, S. W., Parkhill, J., Churcher, C., Dougan, G., Wilson, R. K., Miller, W. (2000). Comparison of the Escherichia coli K-12 genome with sampled genomes of a Klebsiella pneumoniae and three Salmonella enterica serovars, Typhimurium, Typhi and Paratyphi. Nucleic Acids Res 28: 4974-4986 [Abstract] [Full Text]  
  • Daines, D. A., Silver, R. P. (2000). Evidence for Multimerization of Neu Proteins Involved in Polysialic Acid Synthesis in Escherichia coli K1 Using Improved LexA-Based Vectors. J. Bacteriol. 182: 5267-5270 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»