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Journal of Bacteriology, February 2000, p. 696-703, Vol. 182, No. 3
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Alteration of the Lipopolysaccharide Structure Affects the Functioning of the Xcp Secretory System in Pseudomonas aeruginosa

Gérard Michel,1,* Geneviève Ball,1 Joanna B. Goldberg,2 and Andrée Lazdunski1

Laboratoire d'Ingénierie des Systèmes Macromoléculaires, CNRS, 13402 Marseille Cedex 20, France,1 and Department of Microbiology, University of Virginia, Health Sciences Center, Charlottesville, Virginia2

Received 18 June 1999/Accepted 3 November 1999

Pseudomonas aeruginosa secretes a wide range of hydrolytic enzymes into the external medium by the Xcp secretion machinery. To better understand the role played by envelope constituents in the functioning of this type II secretory system, we have studied the influence of lipopolysaccharide (LPS) on the secretion of two extracellular enzymes, the elastase LasB and the lipase LipA. Strains with defective LPS decreased production of LasB and altered the secretion processes of both LasB and LipA without any apparent effect on the composition of the Xcp machinery. The PAO1algC strain, defective in the outer core of LPS, was leaky, as shown by the extracellular release of the periplasmic beta -lactamase. Generation of an xcpR mutation in this mutant led only to a partial accumulation of LasB within the cells, indicating that in strain PAO1algC with a functional xcpR gene, LasB was released in the extracellular medium partly by leakage and partly by secretion. The pool of LasB released into the medium by leakage was not recovered in an active form, while extracellular LasB was active when secreted via the secretory machinery. Further analysis revealed that the presence of a functional Xcp machinery is strictly required for the activation process of LasB. Our results provide evidence that the Xcp system is not fully functional when the LPS structure of P. aeruginosa is altered.

* Corresponding author. Mailing address: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, CNRS, 31 Chemin J. Aiguier, 13402 Marseille Cedex 20, France. Phone: (33) 4-91 16 44 87. Fax: (33) 4 91 71 21 24. E-mail: michel{at}ibsm.cnrs-mrs.fr.

Journal of Bacteriology, February 2000, p. 696-703, Vol. 182, No. 3
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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