OmpR Regulates the Two-Component System SsrA-SsrB in Salmonella Pathogenicity Island 2

doi:10.1128/JB.182.3.771-781.2000

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Journal of Bacteriology, February 2000, p. 771-781, Vol. 182, No. 3
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

OmpR Regulates the Two-Component System SsrA-SsrB in Salmonella Pathogenicity Island 2

Anthea K. Lee,^* Corrella S. Detweiler, and Stanley Falkow

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305

Received 23 August 1999/Accepted 1 November 1999

Salmonella pathogenicity island 2 (SPI-2) encodes a putative, two-component regulatory system, SsrA-SsrB, which regulatesa type III secretion system needed for replication inside macrophagesand systemic infection in mice. The sensor and regulator homologs,ssrAB (spiR), and genes within the secretion system, includingthe structural gene ssaH, are transcribed after Salmonella entershost cells. We have studied the transcriptional regulation ofssrAB and the secretion system by using gfp fusions to the ssrAand ssaH promoters. We found that early transcription of ssrA,after entry into macrophages, is most efficient in the presenceof OmpR. An ompR mutant strain does not exhibit replication withincultured macrophages. Furthermore, footprint analysis shows thatpurified OmpR protein binds directly to the ssrA promoter region.We also show that minimal medium, pH 4.5, induces SPI-2 gene expressionin wild-type but not ompR mutant strains. We conclude that thetype III secretion system of SPI-2 is regulated by OmpR, whichactivates expression of ssrA soon after Salmonella enters themacrophage.

^* Corresponding author. Mailing address: Dept. Microbiology and Immunology, Stanford University School of Medicine, 299 CampusDr., D300, Stanford, CA 94305-5124. Phone: (650) 723-2671. Fax:(650) 723-1837. E-mail: aklee{at}leland.stanford.edu.

Journal of Bacteriology, February 2000, p. 771-781, Vol. 182, No. 3
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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