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Journal of Bacteriology, February 2000, p. 818-820, Vol. 182, No. 3
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Correction of the DNA Sequence of the regB Gene of Rhodobacter capsulatus with Implications for the Membrane Topology of the Sensor Kinase RegB

Weizhen Chen, Andreas Jäger, and Gabriele Klug*

Institut für Mikrobiologie und Molekularbiologie, D-35392 Giessen, Germany

Received 21 July 1999/Accepted 3 November 1999

We corrected the previously published sequence for the regB gene, which encodes a histidine sensor kinase in Rhodobacter capsulatus. The deduced RegB amino acid sequence has an additional putative transmembrane domain at the N terminus. Analysis of RegB-PhoA and RegB-LacZ fusion proteins supports a topology model for RegB with six membrane-spanning domains.

* Corresponding author. Mailing address: Institut für Mikrobiologie und Molekularbiologie, Frankfurter Str. 107, D-35392 Giessen, Germany. Phone: 49 641 99 35542. Fax: 49 641 99 35549. E-mail: Gabriele.Klug{at}mikro.bio.uni-giessen.de.

Journal of Bacteriology, February 2000, p. 818-820, Vol. 182, No. 3
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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