This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sauvonnet, N.
Right arrow Articles by Pugsley, A. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sauvonnet, N.
Right arrow Articles by Pugsley, A. P.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2000, p. 848-854, Vol. 182, No. 3
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

PpdD Type IV Pilin of Escherichia coli K-12 Can Be Assembled into Pili in Pseudomonas aeruginosa

Nathalie Sauvonnet,1 Pierre Gounon,2 and Anthony P. Pugsley1,*

Unité de Génétique Moléculaire (CNRS URA 1773),1 and Station Centrale de Microscopie Électronique,2 Institut Pasteur, 75724 Paris Cedex 15, France

Received 5 August 1999/Accepted 2 November 1999

Escherichia coli K-12 possesses at least 16 chromosomal genes related to genes involved in the formation of type IV pili in other gram-negative bacteria. However, E. coli K-12 does not produce type IV pili when grown under standard laboratory conditions. The results of reverse transcription-PCR, operon fusion analysis, and immunoblotting demonstrated that several of the putative E. coli piliation genes are expressed at very low levels. Increasing the level of expression of the major pilin gene (ppdD) and the linked assembly genes hofB and hofC (homologues of the Pseudomonas aeruginosa type IV pilus assembly genes pilB and pilC) did not lead to pilus production. However, expression of the ppdD gene in P. aeruginosa led to assembly of PpdD into pili that were recognized by antibodies directed against the PpdD protein. Assembly of PpdD into pili in P. aeruginosa was dependent on the expression of the pilB and pilC genes and independent of expression of the P. aeruginosa pilin structural gene pilA.

* Corresponding author. Mailing address: Unité de Génétique moléculaire (CNRS URA 1773), Institut Pasteur, 25, rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: 33-1-45688494. Fax: 33-1-45688960. E-mail: max{at}pasteur.fr.

Journal of Bacteriology, February 2000, p. 848-854, Vol. 182, No. 3
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sinha, S., Cameron, A. D. S., Redfield, R. J. (2009). Sxy Induces a CRP-S Regulon in Escherichia coli. J. Bacteriol. 191: 5180-5195 [Abstract] [Full Text]  
  • Salomonsson, E., Forsberg, A., Roos, N., Holz, C., Maier, B., Koomey, M., Winther-Larsen, H. C. (2009). Functional analyses of pilin-like proteins from Francisella tularensis: complementation of type IV pilus phenotypes in Neisseria gonorrhoeae. Microbiology 155: 2546-2559 [Abstract] [Full Text]  
  • Epstein, E. A., Reizian, M. A., Chapman, M. R. (2009). Spatial Clustering of the Curlin Secretion Lipoprotein Requires Curli Fiber Assembly. J. Bacteriol. 191: 608-615 [Abstract] [Full Text]  
  • Xicohtencatl-Cortes, J., Monteiro-Neto, V., Saldana, Z., Ledesma, M. A., Puente, J. L., Giron, J. A. (2009). The Type 4 Pili of Enterohemorrhagic Escherichia coli O157:H7 Are Multipurpose Structures with Pathogenic Attributes. J. Bacteriol. 191: 411-421 [Abstract] [Full Text]  
  • Fernandes, P. J., Guo, Q., Donnenberg, M. S. (2007). Functional Consequences of Sequence Variation in Bundlin, the Enteropathogenic Escherichia coli Type IV Pilin Protein. Infect. Immun. 75: 4687-4696 [Abstract] [Full Text]  
  • Winther-Larsen, H. C., Wolfgang, M. C., van Putten, J. P. M., Roos, N., Aas, F. E., Egge-Jacobsen, W. M., Maier, B., Koomey, M. (2007). Pseudomonas aeruginosa Type IV Pilus Expression in Neisseria gonorrhoeae: Effects of Pilin Subunit Composition on Function and Organelle Dynamics. J. Bacteriol. 189: 6676-6685 [Abstract] [Full Text]  
  • Li, J., Attila, C., Wang, L., Wood, T. K., Valdes, J. J., Bentley, W. E. (2007). Quorum Sensing in Escherichia coli Is Signaled by AI-2/LsrR: Effects on Small RNA and Biofilm Architecture. J. Bacteriol. 189: 6011-6020 [Abstract] [Full Text]  
  • Cameron, A. D. S., Redfield, R. J. (2006). Non-canonical CRP sites control competence regulons in Escherichia coli and many other {gamma}-proteobacteria. Nucleic Acids Res 34: 6001-6014 [Abstract] [Full Text]  
  • Palchevskiy, V., Finkel, S. E. (2006). Escherichia coli Competence Gene Homologs Are Essential for Competitive Fitness and the Use of DNA as a Nutrient. J. Bacteriol. 188: 3902-3910 [Abstract] [Full Text]  
  • Touhami, A., Jericho, M. H., Boyd, J. M., Beveridge, T. J. (2006). Nanoscale Characterization and Determination of Adhesion Forces of Pseudomonas aeruginosa Pili by Using Atomic Force Microscopy. J. Bacteriol. 188: 370-377 [Abstract] [Full Text]  
  • Lee, M.-S., Chen, L.-Y., Leu, W.-M., Shiau, R.-J., Hu, N.-T. (2005). Associations of the Major Pseudopilin XpsG with XpsN (GspC) and Secretin XpsD of Xanthomonas campestris pv. campestris Type II Secretion Apparatus Revealed by Cross-linking Analysis. J. Biol. Chem. 280: 4585-4591 [Abstract] [Full Text]  
  • Webb, J. S., Lau, M., Kjelleberg, S. (2004). Bacteriophage and Phenotypic Variation in Pseudomonas aeruginosa Biofilm Development. J. Bacteriol. 186: 8066-8073 [Abstract] [Full Text]  
  • Durand, E., Bernadac, A., Ball, G., Lazdunski, A., Sturgis, J. N., Filloux, A. (2003). Type II Protein Secretion in Pseudomonas aeruginosa: the Pseudopilus Is a Multifibrillar and Adhesive Structure. J. Bacteriol. 185: 2749-2758 [Abstract] [Full Text]  
  • Welch, R. A., Burland, V., Plunkett, G. III, Redford, P., Roesch, P., Rasko, D., Buckles, E. L., Liou, S.-R., Boutin, A., Hackett, J., Stroud, D., Mayhew, G. F., Rose, D. J., Zhou, S., Schwartz, D. C., Perna, N. T., Mobley, H. L. T., Donnenberg, M. S., Blattner, F. R. (2002). Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli. Proc. Natl. Acad. Sci. USA 99: 17020-17024 [Abstract] [Full Text]  
  • Finkel, S. E., Kolter, R. (2001). DNA as a Nutrient: Novel Role for Bacterial Competence Gene Homologs. J. Bacteriol. 183: 6288-6293 [Abstract] [Full Text]  
  • Pouttu, R., Westerlund-Wikstrom, B., Lang, H., Alsti, K., Virkola, R., Saarela, U., Siitonen, A., Kalkkinen, N., Korhonen, T. K. (2001). matB, a Common Fimbrillin Gene of Escherichia coli, Expressed in a Genetically Conserved, Virulent Clonal Group. J. Bacteriol. 183: 4727-4736 [Abstract] [Full Text]  
  • Pugsley, A. P., Bayan, N., Sauvonnet, N. (2001). Disulfide Bond Formation in Secreton Component PulK Provides a Possible Explanation for the Role of DsbA in Pullulanase Secretion. J. Bacteriol. 183: 1312-1319 [Abstract] [Full Text]  
  • van Doorn, J., Hollinger, T. C., Oudega, B. (2001). Analysis of the Type IV Fimbrial-Subunit Gene fimA of Xanthomonas hyacinthi: Application in PCR-Mediated Detection of Yellow Disease in Hyacinths. Appl. Environ. Microbiol. 67: 598-607 [Abstract] [Full Text]  
  • Possot, O. M., Vignon, G., Bomchil, N., Ebel, F., Pugsley, A. P. (2000). Multiple Interactions between Pullulanase Secreton Components Involved in Stabilization and Cytoplasmic Membrane Association of PulE. J. Bacteriol. 182: 2142-2152 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»