Journal of Bacteriology, February 2000, p. 855-858, Vol. 182, No. 3
Department of Microbiology and Immunology,
Queen's University, Kingston, Ontario, Canada K7L 3N6
Received 4 August 1999/Accepted 11 November 1999
Methanococcus voltae is a mesophilic archaeon with
flagella composed of flagellins that are initially made with 11- or
12-amino-acid leader peptides that are cleaved prior to incorporation
of the flagellin into the growing filament. Preflagellin peptidase
activity was demonstrated in immunoblotting experiments with flagellin antibody to detect unprocessed and processed flagellin subunits. Escherichia coli membranes containing the expressed
M. voltae preflagellin (as the substrate) were combined in
vitro with methanogen membranes (as the enzyme source). Correct
processing of the preflagellin to the mature flagellin was also shown
directly by comparison of the N-terminal sequences of the two flagellin
species. M. voltae preflagellin peptidase activity was
optimal at 37°C and pH 8.5 and in the presence of 0.4 M KCl with
0.25% (vol/vol) Triton X-100.
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Posttranslational Processing of Methanococcus
voltae Preflagellin by Preflagellin Peptidases of M. voltae and Other Methanogens
*
Corresponding author. Mailing address: Department of
Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada. Phone: (613) 533-2456. Fax: (613) 533-6796. E-mail: jarrellk{at}post.queensu.ca.
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