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Journal of Bacteriology, February 2000, p. 1154-1157, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Covalent Linkage of Polyamines to Peptidoglycan in Anaerovibrio lipolytica

Takashi Hirao,1 Masahide Sato,1 Akira Shirahata,2 and Yoshiyuki Kamio1,*

Laboratory of Applied Microbiology, Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Amamiya-machi, Aoba-ku, Sendai 981-8555, Japan,1 and Department of Biochemistry, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama 350-0295, Japan2

Received 28 September 1999/Accepted 23 November 1999

Spermidine and cadaverine were found to be constituents of the cell wall peptidoglycan of Anaerovibrio lipolytica, a strictly anaerobic bacterium. The peptidoglycan was degraded with the N-acetylmuramyl-L-alanine amidase and endopeptidase into two peptide fragments, peptide I and peptide II, at a molar ratio of 4:1. Peptides I and II were identified as L-alanine-D-glutamic acid(alpha cadaverine)gamma meso-diaminopimelic acid (DAP)-D-alanine and L-alanine-D-glutamic acid(alpha spermidine)gamma meso-DAP-D-alanine, respectively. The N1-amino group of spermidine was linked to the alpha -carboxyl group of the D-glutamic acid residue of peptide II.

* Corresponding author. Mailing address: Laboratory of Applied Microbiology, Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumi-dori, Amamiya-machi, Aoba-ku, Sendai 981-8555, Japan. Phone: 81-22-717-8779. Fax: 81-22-717-8780. E-mail: ykamio{at}biochem.tohoku.ac.jp.

Journal of Bacteriology, February 2000, p. 1154-1157, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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