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Journal of Bacteriology, February 2000, p. 1158-1161, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cell Cycle Arrest in Archaea by the Hypusination Inhibitor N1-Guanyl-1,7-Diaminoheptane

B. P. Mattias Jansson,1,2 Laurence Malandrin,2 and Hans E. Johansson1,2,*

Department of Genetics and Pathology,1 and Department of Cell and Molecular Biology,2 Uppsala University, Uppsala, Sweden

Received 27 September 1999/Accepted 24 November 1999

Hypusination is an essential posttranslational modification unique to archaeal and eukaryotic protein synthesis initiation factor 5A (aIF5A and eIF5A, respectively). We have investigated the effect of the efficient hypusination inhibitor N1-guanyl-1,7-diaminoheptane (GC7) on four archaeal and one bacterial species. We found that (i) archaea are sensitive to GC7, whereas the bacterium Escherichia coli is not, (ii) GC7 causes rapid and reversible arrest of growth of the archaeon Sulfolobus acidocaldarius, and (iii) the growth arrest is accompanied by a specific reversible arrest of the cell cycle prior to cell division. Our findings establish a link between hypusination and sustained growth of archaea and thereby provide the framework to study molecular details of archaeal cell cycle in connection with in vivo functions of hypusine and of aIF5A and eIF5A.

* Corresponding author. Mailing address: Department of Cell and Molecular Biology, Uppsala University, Box 596, S-751 24 Uppsala, Sweden. Phone: 46-18-471-45 65. Fax: 46-18-18-53 03 96. E-mail: Hans.Johansson{at}icm.uu.se.

Journal of Bacteriology, February 2000, p. 1158-1161, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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