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Journal of Bacteriology, February 2000, p. 1167-1171, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Increase of External Osmolarity Reduces Morphogenetic Defects and Accumulation of Chitin in a gas1 Mutant of Saccharomyces cerevisiae

Anna Turchini, Laura Ferrario, and Laura Popolo*

Dipartimento di Fisiologia e Biochimica Generali, Universitá degli Studi di Milano, 20133 Milan, Italy

Received 18 October 1999/Accepted 29 November 1999

We have performed a physiological analysis of the effects of high-osmolarity media on gas1Delta cells. The reductions in the duplication time, number of pluribudded cells, hypersensitivity to Calcofluor and sodium dodecyl sulfate, and chitin level indicate a partial suppression of the mutant phenotype. GAS1 deletion was found to be lethal in the absence of the Bck1 and Slt2 (Mpk1) proteins of the cell integrity pathway.

* Corresponding author. Mailing address: Dipartimento di Fisiologia e Biochimica Generali, Universitá degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy. Phone: +39(2)70644808. Fax: 39(2)70632811. E-mail: Laura.Popolo{at}unimi.it.

Journal of Bacteriology, February 2000, p. 1167-1171, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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