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Journal of Bacteriology, February 2000, p. 1185-1189, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

High-Resolution Physical Map of the Sinorhizobium meliloti 1021 pSyma Megaplasmid

Frederique Barloy-Hubler,1 Delphine Capela,1,2 Melanie J. Barnett,3 Sue Kalman,4 Nancy A. Federspiel,4 Sharon R. Long,3,5 and Francis Galibert1,*

Laboratoire de Recombinaisons Génétiques UPR41-CNRS, Faculté de Médecine, F-35043 Rennes Cedex,1 and Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes UMR215 INRA-CNRS, F-31326 Castanet Tolosan,2 France, and Department of Biological Sciences,3 DNA Sequencing and Technology Center,4 and Howard Hughes Medical Institute,5 Stanford University, Stanford, California 94305

Received 19 July 1999/Accepted 12 November 1999

To facilitate sequencing of the Sinorhizobium meliloti 1021 pSyma megaplasmid, a high-resolution map was constructed by ordering 113 overlapping bacterial artificial chromosome clones with 192 markers. The 157 anonymous sequence tagged site markers (81,072 bases) reveal hypothetical functions encoded by the replicon.

* Corresponding author. Mailing address: Laboratoire de Recombinaisons Génétiques UPR41-CNRS, Faculté de Médecine, 2 Avenue du Professeur Léon Bernard, F-35043 Rennes Cedex, France. Phone: 33 (0)2-99-33-62-16. Fax: 33 (0)2-99-33-62-00. E-mail: francis.galibert{at}univ-rennes1.fr.

Journal of Bacteriology, February 2000, p. 1185-1189, Vol. 182, No. 4
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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