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Journal of Bacteriology, March 2000, p. 1243-1250, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

KorSA from the Streptomyces Integrative Element pSAM2 Is a Central Transcriptional Repressor: Target Genes and Binding Sites

Guennadi Sezonov,dagger Christophe Possoz, Annick Friedmann, Jean-Luc Pernodet, and Michel Guérineau*

Laboratoire de Biologie et Génétique Moléculaire, Institut de Génétique et Microbiologie, UMR CNRS 8621, Université Paris-Sud, 91405 Orsay, France

Received 15 July 1999/Accepted 29 November 1999

pSAM2, a 10.9-kb mobile integrative genetic element from Streptomyces ambofaciens, possesses, as do a majority of Streptomyces conjugative plasmids, a kil-kor system associated with its transfer. The kor function of pSAM2 was attributed to the korSA gene, but its direct role remained unclear. The present study was focused on the determination of the KorSA targets. It was shown that KorSA acts as a transcriptional repressor by binding to a conserved 17-nucleotide sequence found upstream of only two genes: its own gene, korSA, and pra, a gene positively controlling pSAM2 replication, integration, and excision. A unique feature of KorSA, compared to Kor proteins from other Streptomyces conjugative plasmids, is that it does not directly regulate pSAM2 transfer. KorSA does not bind to the pSAM2 genes coding for transfer and intramycelial spreading. Through the repression of pra, KorSA is able to negatively regulate pSAM2 functions activated by Pra and, consequently, to maintain pSAM2 integrated in the chromosome.

* Corresponding author. Mailing address: Laboratoire de Biologie et Génétique Moléculaire, Institut de Génétique et Microbiologie, UMR CNRS 8621, Bâtiment 400, Université Paris-Sud, 91405 Orsay, France. Phone: 33 (0) 1 69 15 69 17. Fax: 33 (0) 1 69 15 45 44. E-mail: michel.guerineau{at}igmors.u-psud.fr.

dagger Present address: Laboratoire de Génétique Microbienne, Institut Jacques Monod, UMR CNRS 7592, Université Paris VI, 75251, Paris, Cedex 05, France.

Journal of Bacteriology, March 2000, p. 1243-1250, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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