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Journal of Bacteriology, March 2000, p. 1257-1263, Vol. 182, No. 5
Department of Molecular Microbiology and
Institute of Biomembranes,1 and
Department of Plant Ecology and Evolution
Biology,2 Utrecht University, 3584 CH Utrecht,
The Netherlands
Received 18 August 1999/Accepted 7 December 1999
One of the major proteins secreted by Pseudomonas
aeruginosa is a 43-kDa protein, which is cleaved by elastase into
smaller fragments, including a 30-kDa and a 23-kDa fragment. The
N-terminal 23-kDa fragment was previously suggested as corresponding to
a staphylolytic protease and was designated LasD (S. Park and D. R. Galloway, Mol. Microbiol. 16:263-270, 1995). However, the sequence of the gene encoding this 43-kDa protein revealed that the N-terminal half of the protein is homologous to the chitin-binding proteins CHB1
of Streptomyces olivaceoviridis and CBP21 of Serratia
marcescens and to the cellulose-binding protein p40 of
Streptomyces halstedii. Furthermore, a short C-terminal
fragment shows homology to a part of chitinase A of Vibrio
harveyi. The full-length 43-kDa protein could bind chitin and was
thereby protected against the proteolytic activity of
elastase, whereas the degradation products did not bind chitin. The
purified 43-kDa chitin-binding protein had no staphylolytic activity,
and comparison of the enzymatic activities in the extracellular medium
of a wild-type strain and a chitin-binding protein-deficient mutant
indicated that the 43-kDa protein supports neither chitinolytic nor
staphylolytic activity. We conclude that the 43-kDa protein, which was
found to be produced by many clinical isolates of P. aeruginosa, is a chitin-binding protein, and we propose to name
it CbpD (chitin-binding protein D).
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Identification of a Chitin-Binding Protein Secreted
by Pseudomonas aeruginosa
*
Corresponding author. Mailing address: Department of
Molecular Microbiology, Utrecht University, Padualaan 8, 3584 CH
Utrecht, The Netherlands. Phone: 31-30-253 3011. Fax: 31-30-251 3655. E-mail: W.Bitter{at}bio.uu.nl.
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