This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Molle, V.
Right arrow Articles by Buttner, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Molle, V.
Right arrow Articles by Buttner, M. J.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2000, p. 1286-1295, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

WhiD and WhiB, Homologous Proteins Required for Different Stages of Sporulation in Streptomyces coelicolor A3(2)

Virginie Molle,1,* Wendy J. Palframan,1 Kim C. Findlay,2 and Mark J. Buttner1

Departments of Molecular Microbiology1 and Cell Biology,2 John Innes Centre, Colney, Norwich NR4 7UH, United Kingdom

Received 4 October 1999/Accepted 5 December 1999

The whiD locus, which is required for the differentiation of Streptomyces coelicolor aerial hyphae into mature spore chains, was localized by map-based cloning to the overlap between cosmids 6G4 and D63 of the minimal ordered library of Redenbach et al. (M. Redenbach et al., Mol. Microbiol. 21:77-96, 1996). Subcloning and sequencing showed that whiD encodes a homologue of WhiB, a protein required for the initiation of sporulation septation in S. coelicolor. WhiD and WhiB belong to a growing family of small (76- to 112-residue) proteins of unknown biochemical function in which four cysteines are absolutely conserved; all known members of this family are found in the actinomycetes. A constructed whiD null mutant showed reduced levels of sporulation, and those spores that did form were heat sensitive, lysed extensively, and were highly irregular in size, arising at least in part from irregularity in septum placement. The whiD null mutant showed extreme variation in spore cell wall deposition; most spores had uniformly thin (20- to 30-nm) walls, but spore chains were frequently observed in which there was irregular but very pronounced (up to 170 nm) cell wall thickening at the junctions between spores. whiD null mutant spores were frequently partitioned into irregular smaller units through the deposition of additional septa, which were often laid down in several different planes, very close to the spore poles. These "minicompartments" appeared to be devoid of chromosomal DNA. Two whiD promoters, whiDp1 and whiDp2, were identified, and their activities were analyzed during development of wild-type S. coelicolor on solid medium. Both promoters were developmentally regulated; whiDp1 and whiDp2 transcripts were detected transiently, approximately at the time when sporulation septa were observed in the aerial hyphae.

* Corresponding author. Mailing address: Department of Molecular Microbiology, John Innes Centre, Colney, Norwich NR4 7UH, United Kingdom. Phone: (44) 1603-452571. Fax: (44) 1603-456844. E-mail: virginie.molle{at}bbsrc.ac.uk.

Journal of Bacteriology, March 2000, p. 1286-1295, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Salerno, P., Larsson, J., Bucca, G., Laing, E., Smith, C. P., Flardh, K. (2009). One of the Two Genes Encoding Nucleoid-Associated HU Proteins in Streptomyces coelicolor Is Developmentally Regulated and Specifically Involved in Spore Maturation. J. Bacteriol. 191: 6489-6500 [Abstract] [Full Text]  
  • Haiser, H. J., Yousef, M. R., Elliot, M. A. (2009). Cell Wall Hydrolases Affect Germination, Vegetative Growth, and Sporulation in Streptomyces coelicolor. J. Bacteriol. 191: 6501-6512 [Abstract] [Full Text]  
  • Guo, M., Feng, H., Zhang, J., Wang, W., Wang, Y., Li, Y., Gao, C., Chen, H., Feng, Y., He, Z.-G. (2009). Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res 19: 1301-1308 [Abstract] [Full Text]  
  • Mistry, B. V., Del Sol, R., Wright, C., Findlay, K., Dyson, P. (2008). FtsW Is a Dispensable Cell Division Protein Required for Z-Ring Stabilization during Sporulation Septation in Streptomyces coelicolor. J. Bacteriol. 190: 5555-5566 [Abstract] [Full Text]  
  • Ventura, M., Canchaya, C., Tauch, A., Chandra, G., Fitzgerald, G. F., Chater, K. F., van Sinderen, D. (2007). Genomics of Actinobacteria: Tracing the Evolutionary History of an Ancient Phylum. Microbiol. Mol. Biol. Rev. 71: 495-548 [Abstract] [Full Text]  
  • Agarwal, N., Raghunand, T. R., Bishai, W. R. (2006). Regulation of the expression of whiB1 in Mycobacterium tuberculosis: role of cAMP receptor protein.. Microbiology 152: 2749-2756 [Abstract] [Full Text]  
  • Hoskisson, P. A., Rigali, S., Fowler, K., Findlay, K. C., Buttner, M. J. (2006). DevA, a GntR-Like Transcriptional Regulator Required for Development in Streptomyces coelicolor.. J. Bacteriol. 188: 5014-5023 [Abstract] [Full Text]  
  • Jakimowicz, P., Cheesman, M. R., Bishai, W. R., Chater, K. F., Thomson, A. J., Buttner, M. J. (2005). Evidence That the Streptomyces Developmental Protein WhiD, a Member of the WhiB Family, Binds a [4Fe-4S] Cluster. J. Biol. Chem. 280: 8309-8315 [Abstract] [Full Text]  
  • Hunt, A. C., Servin-Gonzalez, L., Kelemen, G. H., Buttner, M. J. (2005). The bldC Developmental Locus of Streptomyces coelicolor Encodes a Member of a Family of Small DNA-Binding Proteins Related to the DNA-Binding Domains of the MerR Family. J. Bacteriol. 187: 716-728 [Abstract] [Full Text]  
  • Del Sol, R., Pitman, A., Herron, P., Dyson, P. (2003). The Product of a Developmental Gene, crgA, That Coordinates Reproductive Growth in Streptomyces Belongs to a Novel Family of Small Actinomycete-Specific Proteins. J. Bacteriol. 185: 6678-6685 [Abstract] [Full Text]  
  • Raoult, D., Ogata, H., Audic, S., Robert, C., Suhre, K., Drancourt, M., Claverie, J.-M. (2003). Tropheryma whipplei Twist: A Human Pathogenic Actinobacteria With a Reduced Genome. Genome Res 13: 1800-1809 [Abstract] [Full Text]  
  • Elliot, M. A., Karoonuthaisiri, N., Huang, J., Bibb, M. J., Cohen, S. N., Kao, C. M., Buttner, M. J. (2003). The chaplins: a family of hydrophobic cell-surface proteins involved in aerial mycelium formation in Streptomyces coelicolor. Genes Dev. 17: 1727-1740 [Abstract] [Full Text]  
  • Smith, I. (2003). Mycobacterium tuberculosis Pathogenesis and Molecular Determinants of Virulence. Clin. Microbiol. Rev. 16: 463-496 [Abstract] [Full Text]  
  • Steyn, A. J. C., Collins, D. M., Hondalus, M. K., Jacobs, W. R. Jr., Kawakami, R. P., Bloom, B. R. (2002). Mycobacterium tuberculosis WhiB3 interacts with RpoV to affect host survival but is dispensable for in vivo growth. Proc. Natl. Acad. Sci. USA 99: 3147-3152 [Abstract] [Full Text]  
  • Aínsa, J. A., Ryding, N. J., Hartley, N., Findlay, K. C., Bruton, C. J., Chater, K. F. (2000). WhiA, a Protein of Unknown Function Conserved among Gram-Positive Bacteria, Is Essential for Sporulation in Streptomyces coelicolor A3(2). J. Bacteriol. 182: 5470-5478 [Abstract] [Full Text]  
  • Bibb, M. J., Molle, V., Buttner, M. J. (2000). sigma BldN, an Extracytoplasmic Function RNA Polymerase Sigma Factor Required for Aerial Mycelium Formation in Streptomyces coelicolor A3(2). J. Bacteriol. 182: 4606-4616 [Abstract] [Full Text]  
  • Gomez, J. E., Bishai, W. R. (2000). whmD is an essential mycobacterial gene required for proper septation and cell division. Proc. Natl. Acad. Sci. USA 10.1073/pnas.140225297v1 [Abstract] [Full Text]  
  • Gomez, J. E., Bishai, W. R. (2000). whmD is an essential mycobacterial gene required for proper septation and cell division. Proc. Natl. Acad. Sci. USA 97: 8554-8559 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»