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Journal of Bacteriology, March 2000, p. 1352-1355, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Respiration of 2,4,6-Trinitrotoluene by Pseudomonas sp. Strain JLR11

Abraham Esteve-Nuñez,1,2 Gloria Lucchesi,1,dagger Bodo Philipp,2 Bernhard Schink,2 and Juan L. Ramos1,*

Department of Biochemistry and Molecular and Cellular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, E-18008 Granada, Spain,1 and Department of Biology, University of Konstanz, D-7750 Konstanz, Germany2

Received 2 August 1999/Accepted 7 December 1999

Under anoxic conditions Pseudomonas sp. strain JLR11 can use 2,4,6-trinitrotoluene (TNT) as the sole N source, releasing nitrite from the aromatic ring and subsequently reducing it to ammonium and incorporating it into C skeletons. This study shows that TNT can also be used as a terminal electron acceptor in respiratory chains under anoxic conditions by Pseudomonas sp. strain JLR11. TNT-dependent proton translocation coupled to the reduction of TNT to aminonitrotoluenes has been observed in TNT-grown cells. This extrusion did not occur in nitrate-grown cells or in anaerobic TNT-grown cells treated with cyanide, a respiratory chain inhibitor. We have shown that in a membrane fraction prepared from Pseudomonas sp. strain JLR11 grown on TNT under anaerobic conditions, the synthesis of ATP was coupled to the oxidation of molecular hydrogen and to the reduction of TNT. This phosphorylation was uncoupled by gramicidin. Respiration by Pseudomonas sp. strain JLR11 is potentially useful for the biotreatment of TNT in polluted waters and soils, particularly in phytorhizoremediation, in which bacterial cells are transported to the deepest root zones, which are poor in oxygen.

* Corresponding author. Mailing address: CSIC-Estación Experimental del Zaidín, Profesor Albareda, 1, E-18008 Granada, Spain. Phone: 34-958-121011. Fax: 34-958-129600. E-mail: jlramos{at}eez.csic.es.

dagger Present address: Department of Biochemistry, University of Rio Cuarto, Rio Cuarto, Argentina.

Journal of Bacteriology, March 2000, p. 1352-1355, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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