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Journal of Bacteriology, March 2000, p. 1390-1398, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Regulation of the cnr Cobalt and Nickel Resistance Determinant from Ralstonia sp. Strain CH34dagger

Gregor Grass, Cornelia Große, and Dietrich H. Nies*

Institut für Mikrobiologie, Martin-Luther-Universität Halle-Wittenberg, D-06099 Halle, Germany

Received 19 July 1999/Accepted 16 November 1999

Ralstonia sp. strain CH34 is resistant to nickel and cobalt cations. Resistance is mediated by the cnr determinant located on plasmid pMOL28. The cnr genes are organized in two clusters, cnrYXH and cnrCBA. As revealed by reverse transcriptase PCR and primer extension, transcription from these operons is initiated from promoters located upstream of the cnrY and cnrC genes. These two promoters exhibit conserved sequences at the -10 (CCGTATA) and -35 (CRAGGGGRAG) regions. The CnrH gene product, which is required for expression of both operons, is a sigma factor belonging to the sigma L family, whose activity seems to be governed by the membrane-bound CnrY and CnrX gene products in response to Ni2+. Half-maximal activation from the cnrCBA operon was determined by using appropriate lacZ gene fusions and was shown to occur at an Ni2+ concentration of about 50 µM.

* Corresponding author. Mailing address: Institut für Mikrobiologie, Martin-Luther-Universität Halle-Wittenberg, Kurt-Mothes-Str. 3, 06099 Halle, Germany. Phone: (49)-345-5526352. Fax: (49)-345-5527010. E-mail: d.nies{at}mikrobiologie.uni-halle.de.

dagger This publication is dedicated to Hans G. Schlegel, who started the cnr work, on his 75th birthday.

Journal of Bacteriology, March 2000, p. 1390-1398, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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