Influence of Mutations in the mexR Repressor Gene on Expression of the MexA-MexB-OprM Multidrug Efflux System of Pseudomonas aeruginosa

doi:10.1128/JB.182.5.1410-1414.2000

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Journal of Bacteriology, March 2000, p. 1410-1414, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Influence of Mutations in the mexR Repressor Gene on Expression of the MexA-MexB-OprM Multidrug Efflux System of Pseudomonas aeruginosa

Ramakrishnan Srikumar, Catherine J. Paul, and Keith Poole^*

Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada

Received 18 August 1999/Accepted 30 November 1999

Several nalB-type multidrug-resistant mutants of Pseudomonas aeruginosa overexpressed MexAB-OprM and carried mutations inthe local regulatory gene, mexR. Others, dubbed nalC types, carriedmutations elsewhere and overexpressed MexAB-OprM less extensivelythan the nalB strains. Available evidence showed that MexR actedsolely as repressor. Disruption of the mexR gene at various placessuggested that the 5' end of mexR may be a part of the mexAB-oprMpromoter.

^* Corresponding author. Mailing address: Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L3N6, Canada. Phone: (613) 533-6677. Fax: (613) 533-6796. E-mail:poolek{at}post.queensu.ca.

Journal of Bacteriology, March 2000, p. 1410-1414, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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