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Journal of Bacteriology, March 2000, p. 1432-1436, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Novel Missense Mutations That Affect the Transport Function of MalK, the ATP-Binding-Cassette Subunit of the Salmonella enterica Serovar Typhimurium Maltose Transport System

Sabine Hunke, Heidi Landmesser, and Erwin Schneider*

Institut für Biologie/Bakterienphysiologie, Humboldt-Universität zu Berlin, D-10099 Berlin, Germany

Received 11 August 1999/Accepted 8 December 1999

We report on novel mutations in the malK gene of Salmonella enterica serovar Typhimurium, encoding the ATPase subunit of the maltose transporter (MalFGK2). Biochemical analysis suggests that (i) L86 might be involved in a signaling step during substrate translocation and (ii) E306 may be critical for the structural integrity of the protein.

* Corresponding author. Mailing address: Humboldt-Universität zu Berlin, Institut für Biologie/Bakterienphysiologie, Chausseestr. 117, D-10115 Berlin, Germany. Phone: 49 (0)30-2093-8121. Fax: 49 (0)30-2093-8126. E-mail: erwin.schneider{at}rz.hu-berlin.de.

Journal of Bacteriology, March 2000, p. 1432-1436, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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