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Journal of Bacteriology, March 2000, p. 1437-1441, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Mutational Analysis of Ligand Recognition by Tcp, the Citrate Chemoreceptor of Salmonella enterica Serovar Typhimurium

Tomonori Iwama,1 Ko-Ichiro Nakao,1 Hiroshi Nakazato,2,dagger Shuzo Yamagata,1 Michio Homma,2 and Ikuro Kawagishi2,*

Department of Biotechnology, Division of Utilization of Biological Resources, Faculty of Agriculture, Gifu University, Gifu 501-1193,1 and Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602,2 Japan

Received 8 September 1999/Accepted 8 December 1999

The chemoreceptor Tcp mediates taxis to citrate. To identify citrate-binding residues, we substituted cysteine for seven basic or polar residues that are chosen based on the comparison of Tcp with the well-characterized chemoreceptors. The results suggest that Arg-63, Arg-68, Arg-72, Lys-75, and Tyr-150 (and probably other unidentified residues) are involved in the recognition of citrate.

* Corresponding author. Mailing address: Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan. Phone: 81-52-789-2993. Fax: 81-52-789-3001. E-mail: i45406a{at}nucc.cc.nagoya-u.ac.jp.

dagger Present address: Department of Clinical Pathology, Division of Molecular Cytogenetics, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan.

Journal of Bacteriology, March 2000, p. 1437-1441, Vol. 182, No. 5
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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