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Journal of Bacteriology, March 2000, p. 1492-1498, Vol. 182, No. 6
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The TetA(K) Tetracycline/H+ Antiporter from Staphylococcus aureus: Mutagenesis and Functional Analysis of Motif C

Samantha L. Ginn,dagger Melissa H. Brown, and Ronald A. Skurray*

School of Biological Sciences, University of Sydney, Sydney, New South Wales 2006, Australia

Received 23 September 1999/Accepted 17 December 1999

Conserved motif C, identified within members of the major facilitator superfamily (MFS) of transport proteins that mediate drug export, was examined in the tetracycline resistance efflux protein TetA(K) from Staphylococcus aureus; motif C is contained within transmembrane segment 5. Using site-directed mutagenesis, the importance of the conserved glycine (G151, G155, G159, and G160) and proline (P156) residues within this motif was investigated. Over 40 individual amino acid replacements were introduced; however, only alanine and serine substitutions for glycine at G151, G155, and G160 were found to retain significant levels of tetracycline resistance and transport activity in cells expressing mutant proteins. Notably, P156 and G159 appear to be crucial, as amino acid replacements at these positions either significantly reduced or abolished tetracycline/H+ activity. The highly conserved nature of motif C and its distribution throughout drug exporters imply that the residues of motif C play a similar role in all MFS proteins that function as antiporters.

* Corresponding author. Mailing address: School of Biological Sciences, Macleay Building A12, University of Sydney, Sydney, New South Wales 2006, Australia. Phone: 61 2 9351-2376. Fax: 61 2 9351-4771. E-mail: skurray{at}bio.usyd.edu.au.

dagger Present address: Gene Therapy Research Unit, The New Children's Hospital, Parramatta, New South Wales 2124, Australia.

Journal of Bacteriology, March 2000, p. 1492-1498, Vol. 182, No. 6
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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