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Journal of Bacteriology, March 2000, p. 1754-1756, Vol. 182, No. 6
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

AcrD of Escherichia coli Is an Aminoglycoside Efflux Pump

Emiko Y. Rosenberg,1 Dzwokai Ma,2,dagger and Hiroshi Nikaido1,*

Departments of Molecular and Cell Biology1 and Chemistry,2 University of California, Berkeley, California

Received 14 October 1999/Accepted 16 December 1999

AcrD, a transporter belonging to the resistance-nodulation-division family, was shown to participate in the efflux of aminoglycosides. Deletion of the acrD gene decreased the MICs of amikacin, gentamicin, neomycin, kanamycin, and tobramycin by a factor of two to eight, and Delta acrD cells accumulated higher levels of [3H]dihydrostreptomycin and [3H]gentamicin than did the parent strain.

* Corresponding author. Mailing address: Department of Molecular and Cell Biology, Room 229 Stanley Hall, University of California, Berkeley, CA 94720-3206. Phone: (510) 642-2027. Fax: (510) 643-9290. E-mail: nhiroshi{at}uclink4.berkeley.edu.

dagger Present address: Department of Physiology, School of Medicine, University of California San Francisco, San Francisco, Calif.

Journal of Bacteriology, March 2000, p. 1754-1756, Vol. 182, No. 6
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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