This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hantash, F. M.
Right arrow Articles by Earhart, C. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hantash, F. M.
Right arrow Articles by Earhart, C. F.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2000, p. 1768-1773, Vol. 182, No. 6
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Membrane Association of the Escherichia coli Enterobactin Synthase Proteins EntB/G, EntE, and EntF

Feras M. Hantash and Charles F. Earhart*

Section of Molecular Genetics and Microbiology, The University of Texas at Austin, Austin, Texas 78712-1095

Received 10 May 1999/Accepted 21 December 1999

The cytosolic proteins EntE, EntF, and EntB/G, which are Escherichia coli enzymes necessary for the final stage of enterobactin synthesis, are released by osmotic shock. Here, consistent with the idea that cytoplasmic proteins found in shockates have an affinity for membranes, a small fraction of each was found in membrane preparations. Two procedures demonstrated that the enzymes were enriched in a minor membrane fraction of buoyant density intermediate between that of cytoplasmic and outer membranes, providing indirect support for the notion that these proteins have a role in enterobactin excretion as well as synthesis.

* Corresponding author. Mailing address: Section of Molecular Genetics and Microbiology, The University of Texas at Austin, Austin, TX 78712-1095. Phone: (512) 471-1561. Fax: (512) 471-7088. E-mail: earhart{at}mail.utexas.edu.

Journal of Bacteriology, March 2000, p. 1768-1773, Vol. 182, No. 6
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Douet, V., Expert, D., Barras, F., Py, B. (2009). Erwinia chrysanthemi Iron Metabolism: the Unexpected Implication of the Inner Membrane Platform within the Type II Secretion System. J. Bacteriol. 191: 795-804 [Abstract] [Full Text]  
  • Imperi, F., Putignani, L., Tiburzi, F., Ambrosi, C., Cipollone, R., Ascenzi, P., Visca, P. (2008). Membrane-association determinants of the {omega}-amino acid monooxygenase PvdA, a pyoverdine biosynthetic enzyme from Pseudomonas aeruginosa. Microbiology 154: 2804-2813 [Abstract] [Full Text]  
  • Miethke, M., Marahiel, M. A. (2007). Siderophore-Based Iron Acquisition and Pathogen Control. Microbiol. Mol. Biol. Rev. 71: 413-451 [Abstract] [Full Text]  
  • Lai, J. R., Fischbach, M. A., Liu, D. R., Walsh, C. T. (2006). A protein interaction surface in nonribosomal peptide synthesis mapped by combinatorial mutagenesis and selection. Proc. Natl. Acad. Sci. USA 103: 5314-5319 [Abstract] [Full Text]  
  • Vazquez-Laslop, N., Lee, H., Hu, R., Neyfakh, A. A. (2001). Molecular Sieve Mechanism of Selective Release of Cytoplasmic Proteins by Osmotically Shocked Escherichia coli. J. Bacteriol. 183: 2399-2404 [Abstract] [Full Text]  
  • Saier, M. H. Jr. (2000). Vectorial Metabolism and the Evolution of Transport Systems. J. Bacteriol. 182: 5029-5035 [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»