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Journal of Bacteriology, April 2000, p. 1844-1853, Vol. 182, No. 7
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Identification of Two Novel
hrp-Associated Genes in the hrp Gene Cluster of
Xanthomonas oryzae pv. oryzae
Weiguang
Zhu,
Mark M.
MaGbanua,§ and
Frank F.
White*
Department of Plant Pathology, Kansas State
University, Manhattan, Kansas
Received 15 June 1999/Accepted 3 January 2000
We have cloned a hrp gene cluster from
Xanthomonas oryzae pv. oryzae. Bacteria with mutations in
the hrp region have reduced growth in rice leaves and lose
the ability to elicit a hypersensitive response (HR) on the appropriate
resistant cultivars of rice and the nonhost plant tomato. A 12,165-bp
portion of nucleotide sequence from the presumed left end and extending
through the hrpB operon was determined. The region was most
similar to hrp genes from Xanthomonas
campestris pv. vesicatoria and Ralstonia
solanacearum. Two new hrp-associated loci, named
hpa1 and hpa2, were located beyond the
hrpA operon. The hpa1 gene encoded a 13-kDa
glycine-rich protein with a composition similar to those of harpins and
PopA. The product of hpa2 was similar to lysozyme-like
proteins. Perfect PIP boxes were present in the hrpB and
hpa1 operons, while a variant PIP box was located upstream
of hpa2. A strain with a deletion encompassing
hpa1 and hpa2 had reduced pathogenicity and
elicited a weak HR on nonhost and resistant host plants. Experiments
using single mutations in hpa1 and hpa2
indicated that the loss of hpa1 was the principal cause of
the reduced pathogenicity of the deletion strain. A 1,519-bp insertion
element was located immediately downstream of hpa2.
Hybridization with hpa2 indicated that the gene was present in all of the strains of Xanthomonas examined.
Hybridization experiments with hpa1 and IS1114
indicated that these sequences were detectable in all strains of
X. oryzae pv. oryzae and some other Xanthomonas species.
*
Corresponding author. Mailing address: 4024 Throckmorton Hall, Department of Plant Pathology, Kansas State
University, Manhattan, KS 66506. Phone: (785) 532-6176. Fax: (785)
532-5692. E-mail: fwhite{at}plantpath.ksu.edu.

Contribution number 99-500-J from the Kansas Agriculture Experiment
Station.

Present address: Molecular Cardiology Division, Southwestern
Medical Center, Dallas, TX 75235-8573.
§
Present address: Dept. of Plant Pathology, Univ. of
California-Davis, Davis, CA
95616.
Journal of Bacteriology, April 2000, p. 1844-1853, Vol. 182, No. 7
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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