This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gorbacheva, V. Y.
Right arrow Articles by Cabello, F. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gorbacheva, V. Y.
Right arrow Articles by Cabello, F. C.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2000, p. 2037-2042, Vol. 182, No. 7
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Analysis of the bmp Gene Family in Borrelia burgdorferi Sensu Lato

Victoria Y. Gorbacheva,1 Henry P. Godfrey,2 and Felipe C. Cabello1,*

Departments of Microbiology and Immunology1 and Pathology,2 New York Medical College, Valhalla, New York 10595

Received 6 July 1999/Accepted 4 January 2000

BmpA, BmpB, BmpC, and BmpD are homologous Borrelia burgdorferi lipoproteins of unknown functions, encoded by the bmp genes of paralogous chromosomal gene family 36. At least some of the Bmp proteins are immunogens in infected vertebrate hosts. The genetic organization of the bmp region has been characterized for a variety of B. burgdorferi sensu lato strains by Southern hybridization, PCR amplification, and DNA sequencing. All four bmp genes were present in the same relative order in all B. burgdorferi sensu lato low- and high-passage-number isolates. While there were no differences in the relative orders of the bmp genes in these species, variations in DNA sequence in the bmpD-bmpC and bmpC-bmpA intergenic regions were significantly more common than in the corresponding 3' bmpD and bmpC coding regions. The genetic structure of the chromosomal region containing the bmp genes thus appears to be well conserved across different species of B. burgdorferi, but variations in DNA fine structure that prevent PCR primer annealing may occur in this region and make Southern hybridization much more reliable than PCR for detection of the presence of these genes. Our results also suggest that bmp gene products may be used as reagents in the preparation of vaccines and diagnostic assays to protect against and diagnose Lyme disease produced by B. burgdorferi sensu lato.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, New York Medical College, Basic Science Building, Valhalla, NY 10595-1690. Phone: (914) 594-4182. Fax: (914) 594-4176. E-mail: Cabello{at}nymc.edu.

Journal of Bacteriology, April 2000, p. 2037-2042, Vol. 182, No. 7
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Bryksin, A. V., Godfrey, H. P., Carbonaro, C. A., Wormser, G. P., Aguero-Rosenfeld, M. E., Cabello, F. C. (2005). Borrelia burgdorferi BmpA, BmpB, and BmpD Proteins Are Expressed in Human Infection and Contribute to P39 Immunoblot Reactivity in Patients with Lyme Disease. CVI 12: 935-940 [Abstract] [Full Text]  
  • Ramamoorthy, R., McClain, N. A., Gautam, A., Scholl-Meeker, D. (2005). Expression of the bmpB Gene of Borrelia burgdorferi Is Modulated by Two Distinct Transcription Termination Events. J. Bacteriol. 187: 2592-2600 [Abstract] [Full Text]  
  • Caimano, M. J., Eggers, C. H., Hazlett, K. R. O., Radolf, J. D. (2004). RpoS Is Not Central to the General Stress Response in Borrelia burgdorferi but Does Control Expression of One or More Essential Virulence Determinants. Infect. Immun. 72: 6433-6445 [Abstract] [Full Text]  
  • Shin, J. J., Bryksin, A. V., Godfrey, H. P., Cabello, F. C. (2004). Localization of BmpA on the Exposed Outer Membrane of Borrelia burgdorferi by Monospecific Anti-Recombinant BmpA Rabbit Antibodies. Infect. Immun. 72: 2280-2287 [Abstract] [Full Text]  
  • Pinne, M., Ostberg, Y., Comstedt, P., Bergstrom, S. (2004). Molecular analysis of the channel-forming protein P13 and its paralogue family 48 from different Lyme disease Borrelia species. Microbiology 150: 549-559 [Abstract] [Full Text]  
  • Morozova, O. V., Dobrotvorsky, A. K., Livanova, N. N., Tkachev, S. E., Bakhvalova, V. N., Beklemishev, A. B., Cabello, F. C. (2002). PCR Detection of Borrelia burgdorferi Sensu Lato, Tick-Borne Encephalitis Virus, and the Human Granulocytic Ehrlichiosis Agent in Ixodes persulcatus Ticks from Western Siberia, Russia. J. Clin. Microbiol. 40: 3802-3804 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»