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Journal of Bacteriology, April 2000, p. 2043-2047, Vol. 182, No. 7
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Mobilization of Plasmids and Chromosomal DNA Mediated by the SXT Element, a Constin Found in Vibrio cholerae O139

Bianca Hochhut, Joeli Marrero, and Matthew K. Waldor*

Division of Geographic Medicine/Infectious Diseases, New England Medical Center and Tufts University School of Medicine, Boston, Massachusetts 02111

Received 25 October 1999/Accepted 7 January 2000

The Vibrio cholerae SXT element encodes resistance to multiple antibiotics and is a conjugative, self-transmissible, and chromosomally integrating element (a constin). Excision and self-transfer of the SXT element require an element-encoded integrase. We now report that the SXT element can also mobilize the plasmids RSF1010 and CloDF13 in trans as well as chromosomal DNA in an Hfr-like manner. SXT element-mediated mobilization of plasmids and chromosomal DNA, unlike its self-transfer, is not dependent upon excision of the element from the chromosome. These results raise the possibility that the SXT element and other constins play a general role in horizontal gene transfer among gram-negative bacteria.

* Corresponding author. Mailing address: Division of Geographic Medicine/Infectious Diseases, New England Medical Center and Tufts University School of Medicine, NEMC 041, 750 Washington St., Boston, MA 02111. Phone: (617) 636-7618. Fax: (617) 636-5292. E-mail: mwaldor{at}lifespan.org.

Journal of Bacteriology, April 2000, p. 2043-2047, Vol. 182, No. 7
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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