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Journal of Bacteriology, April 2000, p. 2055-2058, Vol. 182, No. 7
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The Sulfur-Regulated Arylsulfatase Gene Cluster of Pseudomonas aeruginosa, a New Member of the cys Regulon

Jörg Hummerjohann,1 Sascha Laudenbach,1 Julia Rétey,1 Thomas Leisinger,1 and Michael A. Kertesz1,2,*

Institute of Microbiology, Swiss Federal Institute of Technology, ETH-Zentrum, CH-8092 Zürich, Switzerland,1 and School of Biological Sciences, University of Manchester, Manchester M13 9PT, England2

Received 14 October 1999/Accepted 11 January 2000

A gene cluster upstream of the arylsulfatase gene (atsA) in Pseudomonas aeruginosa was characterized and found to encode a putative ABC-type transporter, AtsRBC. Mutants with insertions in the atsR or atsB gene were unable to grow with hexyl-, octyl-, or nitrocatecholsulfate, although they grew normally with other sulfur sources, such as sulfate, methionine, and aliphatic sulfonates. AtsRBC therefore constitutes a general sulfate ester transport system, and desulfurization of aromatic and medium-chain-length aliphatic sulfate esters occurs in the cytoplasm. Expression of the atsR and atsBCA genes was repressed during growth with sulfate, cysteine, or thiocyanate. No expression of these genes was observed in the cysB mutant PAO-CB, and the ats genes therefore constitute an extension of the cys regulon in this species.

* Corresponding author. Mailing address: School of Biological Sciences, University of Manchester, Stopford Bldg., Oxford Rd., Manchester M13 9PT, England. Phone: 44-161-2753895. Fax: 44-161-2755656. E-mail: michael.kertesz{at}man.ac.uk.

Journal of Bacteriology, April 2000, p. 2055-2058, Vol. 182, No. 7
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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