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Journal of Bacteriology, April 2000, p. 2068-2076, Vol. 182, No. 8
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Molecular Characterization of Two-Component Systems
of Helicobacter pylori
Dagmar
Beier1,* and
Rainer
Frank2
Theodor-Boveri-Institut für
Biowissenschaften, Lehrstuhl für Mikrobiologie, Universität
Würzburg, D-97074 Würzburg,1 and
Zentrum für Molekularbiologie, Universität
Heidelberg, D-69120 Heidelberg,2 Germany
Received 29 November 1999/Accepted 20 January 2000
Two-component systems are frequently involved in the adaptation of
bacteria to changing environmental conditions at the level of
transcriptional regulation. Here we report the characterization of
members of the two-component systems of the gastric pathogen Helicobacter pylori deduced from the genome sequence of
strain 26695. We demonstrate that the response regulators HP166,
HP1043, and HP1021 have essential functions, as disruption of the
corresponding genes is lethal for the bacteria, irrespective of the
fact that HP1043 and HP1021 have nonconserved substitutions in crucial
amino acids of their receiver domains. An analysis of the in vitro
phosphorylation properties of the two-component proteins demonstrates
that HP244-HP703 and HP165-HP166 are cognate histidine kinase-response
regulator pairs. Furthermore, we provide evidence that the variability
of the histidine kinase HP165 caused by a poly(C) tract of variable length close to the 3' end of open reading frame 165/164 does not
interfere with the kinase activity of the transmitter domain of HP165.
*
Corresponding author. Mailing address:
Theodor-Boveri-Institut für Biowissenschaften, Lehrstuhl
für Mikrobiologie, Universität Würzburg, Am Hubland,
D-97074 Würzburg, Germany. Phone: 49-931-8884421. Fax:
49-931-8884402. E-mail:
d.beier{at}biozentrum.uni-wuerzburg.de.
Journal of Bacteriology, April 2000, p. 2068-2076, Vol. 182, No. 8
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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