Journal of Bacteriology, April 2000, p. 2314-2320, Vol. 182, No. 8
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
andDepartment of Molecular Microbiology1 and Division of Infectious Disease, Department of Pediatrics,2 Washington University School of Medicine, and St. Louis Children's Hospital,3 St. Louis, Missouri 63110
Received 29 October 1999/Accepted 27 January 2000
Yersinia enterocolitica yplA encodes a phospholipase
required for virulence. Virulence genes are often regulated in response to environmental signals; therefore, yplA expression was
examined using a yplA::lacZY
transcriptional fusion. Maximal yplA expression occurred
between pH 6.5 and pH 7.5 and was induced in the mid-logarithmic growth
phase. Potential Fnr, cyclic AMP (cAMP)-cAMP receptor protein (Crp),
and
F regulatory sites were identified in the nucleotide
sequence. Reduction of yplA expression by aeration,
addition of glucose and sucrose, and application of high temperature
and salt is consistent with Fnr-, cAMP-Crp-, and
F-mediated regulation, respectively. Expression of
yplA was reduced in flhDC and fliA
null strains, indicating that yplA is part of the flagellar regulon.
Present address: Department of Food Science and Technology,
University of California, Davis, Davis, CA 95616.
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