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Journal of Bacteriology, April 2000, p. 2314-2320, Vol. 182, No. 8
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
The Yersinia enterocolitica
Phospholipase Gene yplA Is Part of the Flagellar
Regulon
Deborah H.
Schmiel,1
Glenn M.
Young,1,
and
Virginia L.
Miller1,2,3,*
Department of Molecular
Microbiology1 and Division of Infectious
Disease, Department of Pediatrics,2 Washington
University School of Medicine, and St. Louis Children's
Hospital,3 St. Louis, Missouri 63110
Received 29 October 1999/Accepted 27 January 2000
Yersinia enterocolitica yplA encodes a phospholipase
required for virulence. Virulence genes are often regulated in response to environmental signals; therefore, yplA expression was
examined using a yplA::lacZY
transcriptional fusion. Maximal yplA expression occurred
between pH 6.5 and pH 7.5 and was induced in the mid-logarithmic growth
phase. Potential Fnr, cyclic AMP (cAMP)-cAMP receptor protein (Crp),
and
F regulatory sites were identified in the nucleotide
sequence. Reduction of yplA expression by aeration,
addition of glucose and sucrose, and application of high temperature
and salt is consistent with Fnr-, cAMP-Crp-, and
F-mediated regulation, respectively. Expression of
yplA was reduced in flhDC and fliA
null strains, indicating that yplA is part of the flagellar regulon.
*
Corresponding author. Mailing address: Department of
Molecular Microbiology, Washington University School of Medicine,
Campus Box 8230, 660 S. Euclid Ave., St. Louis, MO 63110-1093. Phone: (314) 747-2132. Fax: (314) 747-2135. E-mail:
virginia{at}borcim.wustl.edu.

Present address: Department of Food Science and Technology,
University of California, Davis, Davis, CA
95616.
Journal of Bacteriology, April 2000, p. 2314-2320, Vol. 182, No. 8
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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