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Journal of Bacteriology, April 2000, p. 2314-2320, Vol. 182, No. 8
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The Yersinia enterocolitica Phospholipase Gene yplA Is Part of the Flagellar Regulon

Deborah H. Schmiel,¹ Glenn M. Young,^1, and Virginia L. Miller^1,2,3,*

Department of Molecular Microbiology¹ and Division of Infectious Disease, Department of Pediatrics,² Washington University School of Medicine, and St. Louis Children's Hospital,³ St. Louis, Missouri 63110

Received 29 October 1999/Accepted 27 January 2000

Yersinia enterocolitica yplA encodes a phospholipase required for virulence. Virulence genes are often regulated in response to environmental signals; therefore, yplA expression was examined using a yplA::lacZY transcriptional fusion. Maximal yplA expression occurred between pH 6.5 and pH 7.5 and was induced in the mid-logarithmic growth phase. Potential Fnr, cyclic AMP (cAMP)-cAMP receptor protein (Crp), and ^F regulatory sites were identified in the nucleotide sequence. Reduction of yplA expression by aeration, addition of glucose and sucrose, and application of high temperature and salt is consistent with Fnr-, cAMP-Crp-, and ^F-mediated regulation, respectively. Expression of yplA was reduced in flhDC and fliA null strains, indicating that yplA is part of the flagellar regulon.

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^* Corresponding author. Mailing address: Department of Molecular Microbiology, Washington University School of Medicine, Campus Box 8230, 660 S. Euclid Ave., St. Louis, MO 63110-1093. Phone: (314) 747-2132. Fax: (314) 747-2135. E-mail: virginia{at}borcim.wustl.edu.

Present address: Department of Food Science and Technology, University of California, Davis, Davis, CA 95616.

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Journal of Bacteriology, April 2000, p. 2314-2320, Vol. 182, No. 8
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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