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Journal of Bacteriology, April 2000, p. 2326-2328, Vol. 182, No. 8
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Role of Motility in Adherence to and Invasion of a Fish Cell Line by Vibrio anguillarum

Patricia Ormonde,^1, Per Hörstedt,² Ronan O'Toole,^1, and Debra L. Milton^1,*

Department of Cell and Molecular Biology¹ and Department of Medical Biosciences, Pathology,² Umeå University, S-901 87 Umeå, Sweden

Received 4 October 1999/Accepted 24 January 2000

To understand further the role of the flagellum of Vibrio anguillarum in virulence, invasive and adhesive properties of isogenic motility mutants were analyzed by using a chinook salmon embryo cell line. Adhesion was unaffected but invasion of the cell line was significantly decreased in nonmotile or partially motile mutants, and the chemotactic mutant was hyperinvasive. These results suggest that active motility aids invasion by V. anguillarum, both in vivo and in vitro.

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^* Corresponding author. Mailing address: Department of Cell and Molecular Biology, Umeå University, S-901 87 Umeå, Sweden. Phone: 46-90-785 3782. Fax: 46-90-771420. E-mail: Debra.Milton{at}cmb.umu.se.

Present address: Myriad Genetics, Salt Lake City, UT 84108.

Present address: Department of Biology, Imperial College of Science, Technology, and Medicine, London, England.

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Journal of Bacteriology, April 2000, p. 2326-2328, Vol. 182, No. 8
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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