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Journal of Bacteriology, May 2000, p. 2635-2638, Vol. 182, No. 9
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Site-Specific Serine Incorporation by Lif and Epr into Positions 3 and 5 of the Staphylococcal Peptidoglycan Interpeptide Bridge

Kerstin Ehlert,1 Martin Tschierske,2 Claudio Mori,2 Werner Schröder,1 and Brigitte Berger-Bächi2,*

Bayer AG, PH Research Antiinfectives I, D-42096 Wuppertal, Germany,1 and Institute of Medical Microbiology, University of Zürich, CH-8028 Zürich, Switzerland2

Received 18 October 1999/Accepted 3 February 2000

The FemAB-like factors Lif and Epr confer resistance to glycylglycine endopeptidases lysostaphin and Ale-1, respectively, by incorporating serine residues into the staphylococcal peptidoglycan interpeptide bridges specifically at positions 3 and 5. This required the presence of FemA and/or FemB, in contrast to earlier postulations.

* Corresponding author. Mailing address: Institute of Medical Microbiology, University of Zürich, Gloriastr. 32, Postfach, CH-8028 Zürich, Switzerland. Phone: 41 1 634 26 50. Fax: 41 1 634 49 06. E-mail: bberger{at}immv.unizh.ch.

Journal of Bacteriology, May 2000, p. 2635-2638, Vol. 182, No. 9
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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