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Journal of Bacteriology, May 2000, p. 2664-2667, Vol. 182, No. 9
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Expression of UGA-Containing Mycoplasma Genes in Bacillus subtilis

T. R. Kannan and Joel B. Baseman*

Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78284-7758

Received 3 September 1999/Accepted 16 February 2000

We used Bacillus subtilis to express UGA-containing Mycoplasma genes encoding the P30 adhesin (one UGA) of Mycoplasma pneumoniae and methionine sulfoxide reductase (two UGAs) of Mycoplasma genitalium. Due to natural UGA suppression, these Mycoplasma genes were expressed as full-length protein products, but at relatively low efficiency, in recombinant wild-type Bacillus. The B. subtilis-expressed Mycoplasma proteins appeared as single bands and not as multiple bands compared to expression in recombinant Escherichia coli. Bacillus mutants carrying mutations in the structural gene (prfB) for release factor 2 markedly enhanced the level of readthrough of UGA-containing Mycoplasma genes.

* Corresponding author. Mailing address: Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78284-7758. Phone: (210) 567-3939. Fax: (210) 567-6612. E-mail: BASEMAN{at}UTHSCSA.EDU.

Journal of Bacteriology, May 2000, p. 2664-2667, Vol. 182, No. 9
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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  • Schmidl, S. R., Hames, C., Stulke, J. (2007). Expression of Mycoplasma Proteins Carrying an Affinity Tag in M. pneumoniae Allows Rapid Purification and Circumvents Problems Related to the Aberrant Genetic Code. Appl. Environ. Microbiol. 73: 7799-7801 [Abstract] [Full Text]  
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