Methylotrophic Methylobacterium Bacteria Nodulate and Fix Nitrogen in Symbiosis with Legumes

doi:10.1128/JB.183.1.214-220.2001

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Sy, A.
	Articles by Dreyfus, B.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Sy, A.
	Articles by Dreyfus, B.

Previous Article | Next Article

Journal of Bacteriology, January 2001, p. 214-220, Vol. 183, No. 1
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.1.214-220.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Methylotrophic Methylobacterium Bacteria Nodulate and Fix Nitrogen in Symbiosis with Legumes

Abdoulaye Sy,¹ Eric Giraud,¹ Philippe Jourand,¹ Nelly Garcia,¹ Anne Willems,² Philippe de Lajudie,¹ Yves Prin,¹ Marc Neyra,¹ Monique Gillis,² Catherine Boivin-Masson,¹^,^* and Bernard Dreyfus¹

LSTM, UMR 113 IRD/INRA/AGRO-M/CIRAD, 34398 Montpellier Cedex 5, France,¹ and Laboratorium voor Microbiologie, Universiteit Gent, B-9000, Ghent, Belgium²

Received 30 June 2000/Accepted 3 October 2000

Rhizobia described so far belong to three distinct phylogenetic branches within the $alpha$ -2 subclass of Proteobacteria. Here wereport the discovery of a fourth rhizobial branch involving bacteriaof the Methylobacterium genus. Rhizobia isolated from Crotalarialegumes were assigned to a new species, "Methylobacterium nodulans,"within the Methylobacterium genus on the basis of 16S ribosomalDNA analyses. We demonstrated that these rhizobia facultativelygrow on methanol, which is a characteristic of Methylobacteriumspp. but a unique feature among rhizobia. Genes encoding two keyenzymes of methylotrophy and nodulation, the mxaF gene, encodingthe $alpha$ subunit of the methanol dehydrogenase, and the nodA gene,encoding an acyltransferase involved in Nod factor biosynthesis,were sequenced for the type strain, ORS2060. Plant tests and nodAamplification assays showed that "M. nodulans" is the only nodulatingMethylobacterium sp. identified so far. Phylogenetic sequenceanalysis showed that "M. nodulans" NodA is closely related toBradyrhizobium NodA, suggesting that this gene was acquired byhorizontal genetransfer.

^* Corresponding author. Mailing address: LSTM, TA 10/J, Baillarguet, 34398 Montpellier Cedex 5, France. Phone: (33) 467 593824. Fax: (33) 467 593 802. E-mail: Catherine.Boivin{at}mpl.ird.fr.

Journal of Bacteriology, January 2001, p. 214-220, Vol. 183, No. 1
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.1.214-220.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

Lin, D. X., Wang, E. T., Tang, H., Han, T. X., He, Y. R., Guan, S. H., Chen, W. X. (2008). Shinella kummerowiae sp. nov., a symbiotic bacterium isolated from root nodules of the herbal legume Kummerowia stipulacea. Int. J. Syst. Evol. Microbiol. 58: 1409-1413 [Abstract] [Full Text]
Kalyuzhnaya, M. G., Hristova, K. R., Lidstrom, M. E., Chistoserdova, L. (2008). Characterization of a Novel Methanol Dehydrogenase in Representatives of Burkholderiales: Implications for Environmental Detection of Methylotrophy and Evidence for Convergent Evolution. J. Bacteriol. 190: 3817-3823 [Abstract] [Full Text]
Knief, C., Frances, L., Cantet, F., Vorholt, J. A. (2008). Cultivation-Independent Characterization of Methylobacterium Populations in the Plant Phyllosphere by Automated Ribosomal Intergenic Spacer Analysis. Appl. Environ. Microbiol. 74: 2218-2228 [Abstract] [Full Text]
Sprent, J. I. (2008). 60Ma of legume nodulation. What's new? What's changing?. J Exp Bot 59: 1081-1084 [Abstract] [Full Text]
Elliott, G. N., Chen, W.-M., Bontemps, C., Chou, J.-H., Young, J. P. W., Sprent, J. I., James, E. K. (2007). Nodulation of Cyclopia spp. (Leguminosae, Papilionoideae) by Burkholderia tuberum. ANN BOT (LOND) 100: 1403-1411 [Abstract] [Full Text]
Andam, C. P., Parker, M. A. (2007). Novel Alphaproteobacterial Root Nodule Symbiont Associated with Lupinus texensis. Appl. Environ. Microbiol. 73: 5687-5691 [Abstract] [Full Text]
Chistoserdova, L., Lapidus, A., Han, C., Goodwin, L., Saunders, L., Brettin, T., Tapia, R., Gilna, P., Lucas, S., Richardson, P. M., Lidstrom, M. E. (2007). Genome of Methylobacillus flagellatus, Molecular Basis for Obligate Methylotrophy, and Polyphyletic Origin of Methylotrophy. J. Bacteriol. 189: 4020-4027 [Abstract] [Full Text]
Zurdo-Pineiro, J. L., Rivas, R., Trujillo, M. E., Vizcaino, N., Carrasco, J. A., Chamber, M., Palomares, A., Mateos, P. F., Martinez-Molina, E., Velazquez, E. (2007). Ochrobactrum cytisi sp. nov., isolated from nodules of Cytisus scoparius in Spain. Int. J. Syst. Evol. Microbiol. 57: 784-788 [Abstract] [Full Text]
Aslam, Z., Lee, C. S., Kim, K.-H., Im, W.-T., Ten, L. N., Lee, S.-T. (2007). Methylobacterium jeotgali sp. nov., a non-pigmented, facultatively methylotrophic bacterium isolated from jeotgal, a traditional Korean fermented seafood. Int. J. Syst. Evol. Microbiol. 57: 566-571 [Abstract] [Full Text]
Madhaiyan, M., Kim, B.-Y., Poonguzhali, S., Kwon, S.-W., Song, M.-H., Ryu, J.-H., Go, S.-J., Koo, B.-S., Sa, T.-M. (2007). Methylobacterium oryzae sp. nov., an aerobic, pink-pigmented, facultatively methylotrophic, 1-aminocyclopropane-1-carboxylate deaminase-producing bacterium isolated from rice. Int. J. Syst. Evol. Microbiol. 57: 326-331 [Abstract] [Full Text]
Choi, Y. J., Morel, L., Bourque, D., Mullick, A., Massie, B., Miguez, C. B. (2006). Bestowing Inducibility on the Cloned Methanol Dehydrogenase Promoter (PmxaF) of Methylobacterium extorquens by Applying Regulatory Elements of Pseudomonas putida F1. Appl. Environ. Microbiol. 72: 7723-7729 [Abstract] [Full Text]
Mantelin, S., Saux, M. F.-L., Zakhia, F., Bena, G., Bonneau, S., Jeder, H., de Lajudie, P., Cleyet-Marel, J.-C. (2006). Emended description of the genus Phyllobacterium and description of four novel species associated with plant roots: Phyllobacterium bourgognense sp. nov., Phyllobacterium ifriqiyense sp. nov., Phyllobacterium leguminum sp. nov. and Phyllobacterium brassicacearum sp. nov.. Int. J. Syst. Evol. Microbiol. 56: 827-839 [Abstract] [Full Text]
Sy, A., Timmers, A. C. J., Knief, C., Vorholt, J. A. (2005). Methylotrophic Metabolism Is Advantageous for Methylobacterium extorquens during Colonization of Medicago truncatula under Competitive Conditions. Appl. Environ. Microbiol. 71: 7245-7252 [Abstract] [Full Text]
Wolde-meskel, E., Terefework, Z., Frostegard, A., Lindstrom, K. (2005). Genetic diversity and phylogeny of rhizobia isolated from agroforestry legume species in southern Ethiopia. Int. J. Syst. Evol. Microbiol. 55: 1439-1452 [Abstract] [Full Text]
Trujillo, M. E., Willems, A., Abril, A., Planchuelo, A.-M., Rivas, R., Ludena, D., Mateos, P. F., Martinez-Molina, E., Velazquez, E. (2005). Nodulation of Lupinus albus by Strains of Ochrobactrum lupini sp. nov.. Appl. Environ. Microbiol. 71: 1318-1327 [Abstract] [Full Text]
Jourand, P., Giraud, E., Bena, G., Sy, A., Willems, A., Gillis, M., Dreyfus, B., de Lajudie, P. (2004). Methylobacterium nodulans sp. nov., for a group of aerobic, facultatively methylotrophic, legume root-nodule-forming and nitrogen-fixing bacteria. Int. J. Syst. Evol. Microbiol. 54: 2269-2273 [Abstract] [Full Text]
Van Aken, B., Peres, C. M., Doty, S. L., Yoon, J. M., Schnoor, J. L. (2004). Methylobacterium populi sp. nov., a novel aerobic, pink-pigmented, facultatively methylotrophic, methane-utilizing bacterium isolated from poplar trees (Populus deltoidesxnigra DN34). Int. J. Syst. Evol. Microbiol. 54: 1191-1196 [Abstract] [Full Text]
Gage, D. J. (2004). Infection and Invasion of Roots by Symbiotic, Nitrogen-Fixing Rhizobia during Nodulation of Temperate Legumes. Microbiol. Mol. Biol. Rev. 68: 280-300 [Abstract] [Full Text]
Chen, W.-M., Moulin, L., Bontemps, C., Vandamme, P., Bena, G., Boivin-Masson, C. (2003). Legume Symbiotic Nitrogen Fixation by {beta}-Proteobacteria Is Widespread in Nature. J. Bacteriol. 185: 7266-7272 [Abstract] [Full Text]
Young, J. M., Kuykendall, L. D., Martinez-Romero, E., Kerr, A., Sawada, H. (2003). Classification and nomenclature of Agrobacterium and Rhizobium - a reply to Farrand et al. (2003). Int. J. Syst. Evol. Microbiol. 53: 1689-1695 [Abstract] [Full Text]
van Berkum, P., Terefework, Z., Paulin, L., Suomalainen, S., Lindstrom, K., Eardly, B. D. (2003). Discordant Phylogenies within the rrn Loci of Rhizobia. J. Bacteriol. 185: 2988-2998 [Abstract] [Full Text]
Rivas, R., Velazquez, E., Willems, A., Vizcaino, N., Subba-Rao, N. S., Mateos, P. F., Gillis, M., Dazzo, F. B., Martinez-Molina, E. (2002). A New Species of Devosia That Forms a Unique Nitrogen-Fixing Root-Nodule Symbiosis with the Aquatic Legume Neptunia natans (L.f.) Druce. Appl. Environ. Microbiol. 68: 5217-5222 [Abstract] [Full Text]
Araujo, W. L., Marcon, J., Maccheroni, W. Jr., van Elsas, J. D., van Vuurde, J. W. L., Azevedo, J. L. (2002). Diversity of Endophytic Bacterial Populations and Their Interaction with Xylella fastidiosa in Citrus Plants. Appl. Environ. Microbiol. 68: 4906-4914 [Abstract] [Full Text]
D'Haeze, W., Holsters, M. (2002). Nod factor structures, responses, and perception during initiation of nodule development. Glycobiology 12: 79R-105R [Abstract] [Full Text]
Lidstrom, M. E., Chistoserdova, L. (2002). Plants in the Pink: Cytokinin Production by Methylobacterium. J. Bacteriol. 184: 1818-1818 [Full Text]
Koenig, R. L., Morris, R. O., Polacco, J. C. (2002). tRNA Is the Source of Low-Level trans-Zeatin Production in Methylobacterium spp.. J. Bacteriol. 184: 1832-1842 [Abstract] [Full Text]
Simms, E. L., Taylor, D. L. (2002). Partner Choice in Nitrogen-Fixation Mutualisms of Legumes and Rhizobia. Integr. Comp. Biol. 42: 369-380 [Abstract] [Full Text]
Tan, Z., Hurek, T., Vinuesa, P., Muller, P., Ladha, J. K., Reinhold-Hurek, B. (2001). Specific Detection of Bradyrhizobium and Rhizobium Strains Colonizing Rice (Oryza sativa) Roots by 16S-23S Ribosomal DNA Intergenic Spacer-Targeted PCR. Appl. Environ. Microbiol. 67: 3655-3664 [Abstract] [Full Text]

Appl. Environ. Microbiol.	Infect. Immun.	Eukaryot. Cell
Mol. Cell. Biol.	J. Virol.	Microbiol. Mol. Biol. Rev.
	ALL ASM JOURNALS