This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Han, M.-J.
Right arrow Articles by Lee, S. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Han, M.-J.
Right arrow Articles by Lee, S. Y.

 Previous Article  |  Next Article 

Journal of Bacteriology, January 2001, p. 301-308, Vol. 183, No. 1
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.1.301-308.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Proteome Analysis of Metabolically Engineered Escherichia coli Producing Poly(3-Hydroxybutyrate)

Mee-Jung Han, Sang Sun Yoon, and Sang Yup Lee*

Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, Taejon 305-701, Korea

Received 12 June 2000/Accepted 7 October 2000

Recombinant Escherichia coli strains harboring heterologous polyhydroxyalkanoate (PHA) biosynthesis genes were shown to accumulate unusually large amounts of PHA. In the present study, integrated cellular responses of metabolically engineered E. coli to the accumulation of poly(3-hydroxybutyrate) (PHB) in the early stationary phase were analyzed at the protein level by two-dimensional gel electrophoresis. Out of 20 proteins showing altered expression levels with the accumulation of PHB, 13 proteins were identified with the aid of mass spectrometry. Three heat shock proteins, GroEL, GroES, and DnaK, were significantly up-regulated in PHB-accumulating cells. Proteins which play essential roles in protein biosynthesis were unfavorably influenced by the accumulation of PHB. Cellular demand for the large amount of acetyl coenzyme A and NADPH for the PHB biosynthesis resulted in the increased synthesis of two enzymes of the glycolytic pathway and one enzyme of the Entner-Doudoroff pathway. The expression of the yfiD gene encoding a 14.3-kDa protein, which is known to be produced at low pH, was greatly induced with the accumulation of PHB. Therefore, it could be concluded that the accumulation of PHB in E. coli acted as a stress on the cells, which reduced the cells' ability to synthesize proteins and induced the expression of various protective proteins.

* Corresponding author. Mailing address: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, Taejon 305-701, Korea. Phone: 82-42-869-3930. Fax: 82-42-869-3910. E-mail: leesy{at}mail.kaist.ac.kr.

Journal of Bacteriology, January 2001, p. 301-308, Vol. 183, No. 1
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.1.301-308.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Tessmer, N., Konig, S., Malkus, U., Reichelt, R., Potter, M., Steinbuchel, A. (2007). Heat-shock protein HspA mimics the function of phasins sensu stricto in recombinant strains of Escherichia coli accumulating polythioesters or polyhydroxyalkanoates. Microbiology 153: 366-374 [Abstract] [Full Text]  
  • Han, M.-J., Lee, S. Y. (2006). The Escherichia coli Proteome: Past, Present, and Future Prospects. Microbiol. Mol. Biol. Rev. 70: 362-439 [Abstract] [Full Text]  
  • Kim, S., Nishioka, M., Hayashi, S., Honda, H., Kobayashi, T., Taya, M. (2005). The Gene yggE Functions in Restoring Physiological Defects of Escherichia coli Cultivated under Oxidative Stress Conditions. Appl. Environ. Microbiol. 71: 2762-2765 [Abstract] [Full Text]  
  • Wolfe, A. J. (2005). The Acetate Switch. Microbiol. Mol. Biol. Rev. 69: 12-50 [Abstract] [Full Text]  
  • Han, M.-J., Jeong, K. J., Yoo, J.-S., Lee, S. Y. (2003). Engineering Escherichia coli for Increased Productivity of Serine-Rich Proteins Based on Proteome Profiling. Appl. Environ. Microbiol. 69: 5772-5781 [Abstract] [Full Text]  
  • Lee, J.-H., Lee, D.-E., Lee, B.-U., Kim, H.-S. (2003). Global Analyses of Transcriptomes and Proteomes of a Parent Strain and an L-Threonine-Overproducing Mutant Strain. J. Bacteriol. 185: 5442-5451 [Abstract] [Full Text]  
  • Maehara, A., Taguchi, S., Nishiyama, T., Yamane, T., Doi, Y. (2002). A Repressor Protein, PhaR, Regulates Polyhydroxyalkanoate (PHA) Synthesis via Its Direct Interaction with PHA. J. Bacteriol. 184: 3992-4002 [Abstract] [Full Text]  
  • Wyborn, N. R., Messenger, S. L., Henderson, R. A., Sawers, G., Roberts, R. E., Attwood, M. M., Green, J. (2002). Expression of the Escherichia coli yfiD gene responds to intracellular pH and reduces the accumulation of acidic metabolic end products. Microbiology 148: 1015-1026 [Abstract] [Full Text]  
  • Kirkpatrick, C., Maurer, L. M., Oyelakin, N. E., Yoncheva, Y. N., Maurer, R., Slonczewski, J. L. (2001). Acetate and Formate Stress: Opposite Responses in the Proteome of Escherichia coli. J. Bacteriol. 183: 6466-6477 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»