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Journal of Bacteriology, January 2001, p. 318-327, Vol. 183, No. 1
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.1.318-327.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

phzO, a Gene for Biosynthesis of 2-Hydroxylated Phenazine Compounds in Pseudomonas aureofaciens 30-84

Shannon M. Delaney,1 Dmitri V. Mavrodi,1,2 Robert F. Bonsall,2 and Linda S. Thomashow3,*

School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4234,1 and Department of Plant Pathology,2 and USDA Agricultural Research Service, Root Disease and Biological Control Research Unit,3 Washington State University, Pullman, Washington 99164-6430

Received 10 April 2000/Accepted 9 October 2000

Certain strains of root-colonizing fluorescent Pseudomonas spp. produce phenazines, a class of antifungal metabolites that can provide protection against various soilborne root pathogens. Despite the fact that the phenazine biosynthetic locus is highly conserved among fluorescent Pseudomonas spp., individual strains differ in the range of phenazine compounds they produce. This study focuses on the ability of Pseudomonas aureofaciens 30-84 to produce 2-hydroxyphenazine-1-carboxylic acid (2-OH-PCA) and 2-hydroxyphenazine from the common phenazine metabolite phenazine-1-carboxylic acid (PCA). P. aureofaciens 30-84 contains a novel gene located downstream from the core phenazine operon that encodes a 55-kDa aromatic monooxygenase responsible for the hydroxylation of PCA to produce 2-OH-PCA. Knowledge of the genes responsible for phenazine product specificity could ultimately reveal ways to manipulate organisms to produce multiple phenazines or novel phenazines not previously described.

* Corresponding author. Mailing address: U.S. Department of Agriculture, Agriculture Research Service, Root Disease and Biological Control Unit, Washington State University, P.O. Box 646430, Pullman, WA 99164-6430. Phone: (509) 335-0930. Fax: (509) 335-7674. E-mail: thomasho{at}mail.wsu.edu.

Journal of Bacteriology, January 2001, p. 318-327, Vol. 183, No. 1
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.1.318-327.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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