This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Egland, K. A.
Right arrow Articles by Greenberg, E. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Egland, K. A.
Right arrow Articles by Greenberg, E. P.

 Previous Article  |  Next Article 

Journal of Bacteriology, January 2001, p. 382-386, Vol. 183, No. 1
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.1.382-386.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Quorum Sensing in Vibrio fischeri: Analysis of the LuxR DNA Binding Region by Alanine-Scanning Mutagenesis

Kristi A. Eglanddagger and E. P. Greenberg*

Department of Microbiology and Graduate Program in Molecular Biology, University of Iowa, Iowa City, Iowa 52242

Received 9 June 2000/Accepted 27 September 2000

LuxR is the transcriptional activator for quorum-sensing control of luminescence in Vibrio fischeri. A series of alanine-scanning mutants spanning a predicted helix-turn-helix region in the DNA binding domain of LuxR was constructed, and the activity of each of the LuxR mutant proteins in recombinant Escherichia coli was investigated. The region covered by the mutagenesis spanned residues 190 to 224. About half of the alanine-scanning mutants showed activities similar to that of the wild-type LuxR: at least two were positive-control mutants, four appeared to be defective in DNA binding, and several others were characterized as DNA binding affinity mutants. This analysis, taken together with information about other bacterial transcription factors, provides insights into amino acid residues in LuxR that are involved in DNA binding and transcriptional activation.

* Corresponding author. Mailing address: Department of Microbiology, University of Iowa, Iowa City, IA 52242. Phone: (319) 335-7775. Fax: (319) 335-7949. E-mail: everett-greenberg{at}uiowa.edu.

dagger Present address: Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.

Journal of Bacteriology, January 2001, p. 382-386, Vol. 183, No. 1
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.1.382-386.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Rambow-Larsen, A. A., Rajashekara, G., Petersen, E., Splitter, G. (2008). Putative Quorum-Sensing Regulator BlxR of Brucella melitensis Regulates Virulence Factors Including the Type IV Secretion System and Flagella. J. Bacteriol. 190: 3274-3282 [Abstract] [Full Text]  
  • Haseltine, E. L., Arnold, F. H. (2008). Implications of Rewiring Bacterial Quorum Sensing. Appl. Environ. Microbiol. 74: 437-445 [Abstract] [Full Text]  
  • Hawkins, A. C., Arnold, F. H., Stuermer, R., Hauer, B., Leadbetter, J. R. (2007). Directed Evolution of Vibrio fischeri LuxR for Improved Response to Butanoyl-Homoserine Lactone. Appl. Environ. Microbiol. 73: 5775-5781 [Abstract] [Full Text]  
  • White, C. E, Winans, S. C (2007). Cell-cell communication in the plant pathogen Agrobacterium tumefaciens. Phil Trans R Soc B 362: 1135-1148 [Abstract] [Full Text]  
  • Castang, S., Reverchon, S., Gouet, P., Nasser, W. (2006). Direct Evidence for the Modulation of the Activity of the Erwinia chrysanthemi Quorum-sensing Regulator ExpR by Acylhomoserine Lactone Pheromone. J. Biol. Chem. 281: 29972-29987 [Abstract] [Full Text]  
  • Visick, K. L., Fuqua, C. (2005). Decoding Microbial Chatter: Cell-Cell Communication in Bacteria. J. Bacteriol. 187: 5507-5519 [Full Text]  
  • Chai, Y., Winans, S. C. (2005). Amino-Terminal Protein Fusions to the TraR Quorum-Sensing Transcription Factor Enhance Protein Stability and Autoinducer-Independent Activity. J. Bacteriol. 187: 1219-1226 [Abstract] [Full Text]  
  • Shimane, K., Ogura, M. (2004). Mutational Analysis of the Helix-Turn-Helix Region of Bacillus subtilis Response Regulator DegU, and Identification of cis-Acting Sequences for DegU in the aprE and comK Promoters. J Biochem 136: 387-397 [Abstract] [Full Text]  
  • Urbanowski, M. L., Lostroh, C. P., Greenberg, E. P. (2004). Reversible Acyl-Homoserine Lactone Binding to Purified Vibrio fischeri LuxR Protein. J. Bacteriol. 186: 631-637 [Abstract] [Full Text]  
  • Luo, Z.-Q., Smyth, A. J., Gao, P., Qin, Y., Farrand, S. K. (2003). Mutational Analysis of TraR. CORRELATING FUNCTION WITH MOLECULAR STRUCTURE OF A QUORUM-SENSING TRANSCRIPTIONAL ACTIVATOR. J. Biol. Chem. 278: 13173-13182 [Abstract] [Full Text]  
  • Crater, D. L., Moran, C. P. Jr (2002). Two Regions of GerE Required for Promoter Activation in Bacillus subtilis. J. Bacteriol. 184: 241-249 [Abstract] [Full Text]  
  • Crater, D. L., Moran, C. P. Jr. (2001). Identification of a DNA Binding Region in GerE from Bacillus subtilis. J. Bacteriol. 183: 4183-4189 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»