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Journal of Bacteriology, January 2001, p. 401-404, Vol. 183, No. 1
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.1.401-404.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cloning, Expression, and Characterization of cis-Polyprenyl Diphosphate Synthase from the Thermoacidophilic Archaeon Sulfolobus acidocaldarius

Hisashi Hemmi, Satoshi Yamashita, Takefumi Shimoyama, Toru Nakayama, and Tokuzo Nishino*

Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai, Miyagi 980-8579, Japan

Received 30 May 2000/Accepted 5 October 2000

cis-polyprenyl diphosphate synthases are involved in the biosynthesis of the glycosyl carrier lipid in most organisms. However, only little is known about this enzyme of archaea. In this report, we isolated the gene of cis-polyprenyl diphosphate synthase from a thermoacidophilic archaeon, Sulfolobus acidocaldarius, and characterized the recombinant enzyme.

* Corresponding author. Mailing address: Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai, Miyagi 980-8579, Japan. Phone and fax: 81-22-217-7270. E-mail: nishino{at}mail.cc.tohoku.ac.jp.

Journal of Bacteriology, January 2001, p. 401-404, Vol. 183, No. 1
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.1.401-404.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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