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Journal of Bacteriology, January 2001, p. 405-409, Vol. 183, No. 1
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.1.405-409.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

areCBA Is an Operon in Acinetobacter sp. Strain ADP1 and Is Controlled by AreR, a sigma 54-Dependent Regulator

Rheinallt M. Jones and Peter A. Williams*

School of Biological Sciences, University of Wales Bangor, Bangor, Gwynedd LL57 2UW, Wales, United Kingdom

Received 12 July 2000/Accepted 12 October 2000

The areCBA genes in Acinetobacter sp. strain ADP1, determining growth on benzyl alkanoates, are shown to be transcribed as a single operon and regulated by areR, which encodes a regulatory protein of the NtrC/XylR family. Assays of the Are enzymes and of two insertions of lacZ as a reporter gene have shown that the operon is induced by benzyl acetate, benzyl alcohol, and benzaldehyde, as well as 2- and 4-hydroxybenzyl acetates and benzyl propionate and butyrate. Two adjacent sites of transcriptional initiation were 97 and 96 bp upstream of the start codon for areC, near a sigma 54-dependent -12, -24 promoter. Inactivation of areR and rpoN (for RNA polymerase sigma 54) drastically reduced growth rates on the Are substrates and induction of the operon.

* Corresponding author. Mailing address: School of Biological Sciences, University of Wales Bangor, Bangor, Gwynedd LL57 2UW, Wales, United Kingdom. Phone: (44) 1248 382363. Fax: (44) 1248 370731. E-mail: P.A.Williams{at}bangor.ac.uk.

Journal of Bacteriology, January 2001, p. 405-409, Vol. 183, No. 1
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.1.405-409.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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