Characterization of a Putative Pathogenicity Island from Bovine Staphylococcus aureus Encoding Multiple Superantigens

doi:10.1128/JB.183.1.63-70.2001

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Journal of Bacteriology, January 2001, p. 63-70, Vol. 183, No. 1
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.1.63-70.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of a Putative Pathogenicity Island from Bovine Staphylococcus aureus Encoding Multiple Superantigens

J. Ross Fitzgerald,¹^,^* Steven R. Monday,² Timothy J. Foster,¹ Gregory A. Bohach,² Patrick J. Hartigan,³ William J. Meaney,⁴ and Cyril J. Smyth¹

Department of Microbiology, Moyne Institute of Preventive Medicine,¹ and Department of Physiology,³ Trinity College, Dublin 2, and Dairy Production Research Centre, Teagasc, Moorepark, Fermoy, County Cork,⁴ Republic of Ireland, and Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844²

Received 21 July 2000/Accepted 11 October 2000

Previous studies have demonstrated that a proportion of Staphylococcus aureus isolates from bovine mastitis coproduce toxicshock syndrome toxin (TSST) and staphylococcal enterotoxin C (SEC).In this study, molecular genetic analysis of one such strain,RF122, revealed the presence of a 15,891-bp putative pathogenicityisland (SaPIbov) encoding the genes for TSST (tst), the SEC bovinevariant (sec-bovine), and a gene (sel) which encodes an enterotoxin-likeprotein. The island contains 21 open reading frames specifyinghypothetical proteins longer than 60 amino acids including anintegrase-like gene. The element is bordered by 74-bp direct repeatsat the left and right junctions, and the integration site liesadjacent to the 3' end of the GMP synthase gene (gmps) in theS. aureus chromosome. SaPIbov contains a central region of sequenceidentity with the previously characterized tst pathogenicity islandSaPI1 (J. A. Lindsay et al., Mol. Microbiol. 29:527-543, 1998).A closely related strain, RF120, of the same multilocus enzymeelectrophoretic type, random amplified polymorphic DNA type, andribotype, does not contain the island, implying that the elementis mobile and that a recent insertion/deletion event has takenplace. TSST and TSST/SEC-deficient mutants of S. aureus strainRF122 were constructed by allele replacement. In vitro bovineV $beta$ -specific lymphocyte expansion analysis by culture supernatantsof wild-type strains and of tst and sec-bovine allele replacementmutants revealed that TSST stimulates BTB13-specific T cells whereasSEC-bovine stimulates BTB93-specific T cells. This suggests thatthe presence of SaPIbov may contribute to modulation of the bovineimmuneresponse.

^* Corresponding author. Present address: Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Instituteof Allergy and Infectious Diseases, National Institutes of Health,903 South Fourth St., Hamilton, MT 59840. Phone: (406) 363-9305.Fax: (406) 363-9394. E-mail: rfitzgerald{at}niaid.nih.gov.

Journal of Bacteriology, January 2001, p. 63-70, Vol. 183, No. 1
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.1.63-70.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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