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Journal of Bacteriology, May 2001, p. 3134-3141, Vol. 183, No. 10
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.10.3134-3141.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Osmoregulated Periplasmic Glucan Synthesis Is Required for Erwinia chrysanthemi Pathogenicity

Frederic Page,1 Silvia Altabe,1,dagger Nicole Hugouvieux-Cotte-Pattat,2 Jean-Marie Lacroix,1 Janine Robert-Baudouy,2 and Jean-Pierre Bohin1,*

Unité de Glycobiologie Structurale et Fonctionnelle, UMR USTL-CNRS 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq Cedex,1 and Unité de Microbiologie et Génétique, INSA, ERS INSA-UCB-CNRS 2009, INSA, 69621 Villeurbanne Cedex,2 France

Received 5 December 2000/Accepted 6 March 2001

Erwinia chrysanthemi is a phytopathogenic enterobacterium causing soft rot disease in a wide range of plants. Osmoregulated periplasmic glucans (OPGs) are intrinsic components of the gram-negative bacterial envelope. We cloned the opgGH operon of E. chrysanthemi, encoding proteins involved in the glucose backbone synthesis of OPGs, by complementation of the homologous locus mdoGH of Escherichia coli. OpgG and OpgH show a high level of similarity with MdoG and MdoH, respectively, and mutations in the opgG or opgH gene abolish OPG synthesis. The opg mutants exhibit a pleiotropic phenotype, including overproduction of exopolysaccharides, reduced motility, bile salt hypersensitivity, reduced protease, cellulase, and pectate lyase production, and complete loss of virulence. Coinoculation experiments support the conclusion that OPGs present in the periplasmic space of the bacteria are necessary for growth in the plant host.

* Corresponding author. Mailing address: U.S.T.L., Bât. C9, 59655 Villeneuve d'Ascq Cedex, France. Phone: 34 (0)3 20 43 65 92. Fax: 34 (0)3 20 43 65 55. E-mail: Jean-Pierre.Bohin{at}univ-lille1.fr.

dagger Present address: Departomento de Microbiologia, Facultad de Ciencias Bioquimicos Formacenticas, Universidad Nacional de Rosario UNR Suipocha 531, 2000 Rosario Santa Fe, Argentina.

Journal of Bacteriology, May 2001, p. 3134-3141, Vol. 183, No. 10
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.10.3134-3141.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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