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Journal of Bacteriology, May 2001, p. 3247-3250, Vol. 183, No. 10
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.10.3247-3250.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Functional Complementation of Pyran Ring Formation in Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2) by Ketoreductase Genes for Granaticin Biosynthesis

Koji Ichinose,1,* Takaaki Taguchi,1 David J. Bedford,2 Yutaka Ebizuka,1 and David A. Hopwood2

Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan,1 and Department of Genetics, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom2

Received 15 November 2000/Accepted 26 January 2001

A mutation in actVI-ORF1, which controls C-3 reduction in actinorhodin biosynthesis by Streptomyces coelicolor, was complemented by gra-ORF5 and -ORF6 from the granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tü22. It is hypothesized that, while gra-ORF5 alone is a ketoreductase for C-9, gra-ORF6 gives the enzyme regiospecificity also for C-3.

* Corresponding author. Mailing address: Department of Natural Product Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Phone: 81 (3) 5841-4742. Fax: 81 (3) 5841-4744. E-mail: ichinose{at}mol.f.u.tokyo.ac.jp.

Journal of Bacteriology, May 2001, p. 3247-3250, Vol. 183, No. 10
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.10.3247-3250.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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