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Journal of Bacteriology, May 2001, p. 3251-3255, Vol. 183, No. 10
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.10.3251-3255.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cloning and Expression of Two Genes Coding for Sodium Pumps in the Salt-Tolerant Yeast Debaryomyces hansenii

Anabel Almagro,1,2,dagger Catarina Prista,3 Begoña Benito,2 Maria C. Loureiro-Dias,3 and José Ramos1,*

Departamento de Microbiología, Escuela Técnica Superior de Ingenieros Agrónomos, E-14071 Córdoba,1 and Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, E-28040, Madrid,2 Spain, and Departamento de Botânica e Engenharia Biológica, Instituto Superior de Agronomia, 1349-017 Lisbon, Portugal3

Received 10 November 2000/Accepted 27 February 2001

Two genes encoding Na+-ATPases from Debaryomyces hansenii were cloned and sequenced. The genes, designated ENA1 from D. hansenii (DhENA1) and DhENA2, exhibited high homology with the corresponding genes from Schwanniomyces occidentalis. DhENA1 was expressed in the presence of high Na+ concentrations, while the expression of DhENA2 also required high pH. A mutant of Saccharomyces cerevisiae lacking the Na+ efflux systems and sensitive to Na+, when transformed with DhENA1 or DhENA2, recovered Na+ tolerance and also the ability to extrude Na+.

* Corresponding author. Mailing address: Departamento de Microbiología, Escuela Técnica Superior de Ingenieros Agronomos, E-14071 Cordoba, Spain. Phone: 34 957 218521. Fax: 34 957 218563. E-mail: mi1raruj{at}uco.es.

dagger Present address: Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan.

Journal of Bacteriology, May 2001, p. 3251-3255, Vol. 183, No. 10
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.10.3251-3255.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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